Influence of Mg concentration, pH value and specimen parameter on the hemolytic property of biodegradable magnesium

In this study, we first evaluated the hemolytic rate of pure magnesium and MgZn alloy, and the experimental results indicated that both of these two materials showed severe hemolysis. Furthermore, a revised hemolysis test was designed to assess the impact of the Mg²⁺ concentration and the pH value on the hemolytic rate of magnesium. It was found that a Mg²⁺ concentration of 11.4 ppm in the normal saline extract with a pH value of 7.35 or 4.93 did not demonstrate any hemolysis. However, the extract with the same Mg²⁺ concentration but a pH value of 12.01 or 2.48 could result in heavy hemolysis. In addition, compared to the pervious study, it was suggested that there should be a preferred choice between the mass and the surface area of the specimen that used in the hemolysis test according to its different medical applications.