| 液相色谱分离-飞行时间质谱法鉴定中药三七中主要皂苷化合物 |
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| 引用本文： | 何,弦,唐晓花,闫海全,李崇瑛.液相色谱分离-飞行时间质谱法鉴定中药三七中主要皂苷化合物[J].四川化工,2014(2):32-35. |
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| 作者姓名： | 何 弦 唐晓花 闫海全 李崇瑛 |
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| 作者单位： | [1]成都理工大学材料与化学化工学院,四川成都610059 [2]广西钦州市钦南区沙埠中学,广西钦州535000 [3]四川省泸州市环境保护监测站,四川泸州646000 |
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| 摘 要： | 采用Kromasil C18(4.6mm×200mm,5μm)色谱柱,1mL/min流速,202nm检测波长,室温,乙腈-水二元梯度洗脱,以及负离子模式,0.34MPa雾化器压力,3600V毛细管电压,100V碎片器电压,10L/min氮气流量,300℃氮气温度。实现了广西产三七总皂苷样品中20多种化合物的液相色谱分离及多个皂苷化合物的飞行时间质谱鉴定。质谱鉴定结果表明广西三七的主要皂苷化合物有人参皂苷Rg1、人参皂苷Re、三七皂苷R1、人参皂苷Rb1、人参皂苷Rd,是三七中的上品,既可入药也可食补。
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| 关 键 词： | 广西三七 皂苷 高效液相色谱-飞行时间质谱 |
HPLC Separation-TOF/MS Identification of Saponins in Panax Notoginseng |
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| He Xian,Tang Xiaohua,Yan Haiquan,Li Chongying.HPLC Separation-TOF/MS Identification of Saponins in Panax Notoginseng[J].sichuan chemical industry,2014(2):32-35. |
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| Authors: | He Xian Tang Xiaohua Yan Haiquan Li Chongying |
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| Affiliation: | 1. College of Materials and Chemistry ＆Chemical Engineering ,Chengdu University of Technology, Chengdu 610059, Sichuan, China ; 2. Shabu School in Qinzhou of Guangxi, Qinzhou 535000, Guangxi, China 3. Luzhou Environmental Protection Monitoring Station, Luzhou 646000, Sichuan,China) |
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| Abstract: | HPLC separation and TOF-MS identification methods of saponins of the Panax Notoginseng were established with the HPLC conditions of Kromasil C18 （4.6mm×200mm,5μm）, flow rate at lmL/min, detection wavelength at 202nm, room temperature and mobile phase of acetonitrile-water with gradient e- lution, and the MS conditions of the negative ion mode, drying gas （nitrogen） temperature at 350℃, dr- ying gas flow at 10 L/min, nebulizer pressure at 0. 34MPa, capillary voltage （Vcap） at 4000 V, fragmen- tor voltage at 100 V. The results indicated that the separation was satisfactary and five saponins were iden- tified in the Panax Notoginseng. |
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| Keywords: | Panax Notoginseng saponin HPLC-TOF/MS |
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