HPLC analysis of phospholipids by evaporative laser light-scattering detection |
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| Authors: | T L Mounts S L Abidl K A Rennick |
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| Affiliation: | (1) Food Quality and Safety Research, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 61604 Peoria, Illinois |
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| Abstract: | High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection
of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative
analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at
high moisture content. Analytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major
class fractions were analyzed by transmethylation and capillary collumn chromatography for fatty acid composition, and by
reverse-phase C-18 HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of
phosphatidylcholine and phosphatidylethanolamine. |
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| Keywords: | Capillary gas chromatography crude soybean oil damaged soybeans degummed soybean oil high performance liquid chromatography phospholipids |
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