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| 纳米TiO2光诱导杀伤 Bel-7402人体肝癌细胞研究 | |
| 引用本文： | 夏春辉,王百齐,王玉,刘亚琴,徐广有,吴山. 纳米TiO2光诱导杀伤 Bel-7402人体肝癌细胞研究[J]. 无机材料学报, 2006, 21(6): 1467-1471. DOI: 10.3724/SP.J.1077.2006.01467 |
| 作者姓名： | 夏春辉 王百齐 王玉 刘亚琴 徐广有 吴山 |
| 作者单位： | 1. 齐齐哈尔医学院化学教研室,齐齐哈尔,161042 2. 哈尔滨工业大学应用化学系,哈尔滨,150001 3. 齐齐哈尔医学院化学教研室,齐齐哈尔,161042;中国医科大学生物化学教研室,沈阳,110001 |
| 基金项目： | 黑龙江省高校骨干教师创新能力资助计划;黑龙江省杰出青年科学基金;黑龙江省教育厅科学技术研究项目;齐齐哈尔医学院校科研和教改项目 |
| 摘 要： | 在光诱导条件下，采用HE染色法和四甲基偶氮唑蓝比色法（MTT法），研究了纳米TiO2对Bel-7402人体肝癌细胞的杀伤作用，考察了在不同纳米TiO2浓度、不同光照时间下纳米TiO2的抑瘤效果，并且对抑瘤机制进行了探讨．结果发现：在光诱导条件下，适宜的TiO2浓度具有较高的抑瘤率，同时抑瘤过程表现出类似一级反应的动力学规律；在光诱导条件下，纳米TiO2产生的活性氧组分与癌细胞膜内外的生物大分子反应，引起广泛的细胞结构破坏；造成癌细胞内Ca^2＋离子稳态失去平衡；引发细胞微管相关蛋白2（MAP-2）表达的变化，促进微管发生重组，从而导致细胞凋亡和坏死． |
| 关 键 词： | 纳米TiO2 光诱导杀伤 肝癌细胞 |
| 文章编号： | 1000-324X（2006）06-1467-05 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005-12-192006-03-16 |
Damaging Effects of Nanosized TiO2 on Bel-7402 Human Liver Cancer Cell Under Photoinduce |
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| XIA Chun-Hui,WANG Bai-Qi,WANG Yu,LIU Ya-Qin,XU Guang-You,WU Shan. Damaging Effects of Nanosized TiO2 on Bel-7402 Human Liver Cancer Cell Under Photoinduce[J]. Journal of Inorganic Materials, 2006, 21(6): 1467-1471. DOI: 10.3724/SP.J.1077.2006.01467 | |
| Authors: | XIA Chun-Hui WANG Bai-Qi WANG Yu LIU Ya-Qin XU Guang-You WU Shan |
| Affiliation: | 1. Chemistry Department, Qiqihaer Medical College, Qiqihaer 161042, China; 2. Department of Applied Chemistry, Harbin Institute of Technology, Harbin 150001, China; 3. Department of Biological Chemistry, China Medical University, Shenyang 110001, China |
| Abstract: | The damaging effects of nanosized TiO_2 on human Bel-7402 liver cancer cell were investigated by means of HE dye method and MTT colorimetric assay under condition of photoin- duce.The influences of nanosized TiO_2 content,and irradiation time on cancer inhibition were systematically studied,and the inhibition mechanism was primarily discussed.The results show that nanosized TiO_2 exhibits good inhibition effects under appropriate TiO_2 concentration,and the inhibition process obeys first order reaction rule approximately;In addition,activity oxygen produced by nanosized TiO_2 under illumination can react with organicmolecules in and out the cancer cell membrane,so that lead to damage cell structure,result into imbalance of Ca~(2+) in the cancer cell,and induce microtubule reassembly due to expression changes of microtubule-associated protein-2,which are responsible for the apoptotic and dead cancer cells. |
| Keywords: | MAP-2 |
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