超高效液相色谱法检测焙烤食品馅料中的 二氧化硫脲

目的 建立馅料中二氧化硫脲的超高效液相色谱测定方法。方法 用0.05%乙酸水溶液提取,样品提取液经过离心,乙腈稀释过滤膜过滤后,以HILIC色谱柱为分析柱分离,采用超高效液相色谱-二极管阵列检测器(UPLC - PDA)检测,以乙腈和水为流动相,梯度洗脱,检测波长为284 nm。采用外标法定量。结果 二氧化硫脲的线性范围为0.4 ~ 20 mg/L,相关系数不小于0.999,空白样品的加标回收的回收率为80.6% ~ 102.2%,相对标准偏差为2.6% ~ 5.0%。结论 该方法简便、快速、准确、重现性好,适用于馅料中二氧化硫脲的检测。

Determination of thiourea dioxide in fillings of bakery food by ultra performance liquid chromatography

Objective To establish a method to detect the content of thiourea dioxide in fillings of bakery food by using Ultra Performance Liquid Chromatography. Methods After the thiourea dioxide was extracted by 0.05% acetic acid, and then centrifuged. The extracts was separated by HILIC column, and detected by the Photo-Diode Array(PDA) at the wavelength of 284 nm. Results The thiourea dioxide concentration and the peak area showed good linearity from 0.4 to 20 mg/L with the correlation (r) not less than 0.999. The average recoveries of the spiked blank fillings of bakery food were from 80.6% to 102.2% with the relative standard deviations (RSD) in the range of 2.6% - 5.0%. Conclusion The method is simple, quick, accurate and robust for the detection of thiourea dioxide in fillings of bakery food.