两种检测SOD酶活性方法的比较

目的比较邻苯三酚自氧化法和氮蓝四唑(NBT)光还原法分别测定超氧化物歧化酶(SOD)酶活力的差异。方法采用两种方法分别测定SOD标准品、苜蓿和番茄两种植物提取物的不同来源SOD酶活性,比较其精确性和灵敏度。结果在标准品SOD酶活的测定中,邻苯三酚自氧化法和NBT光还原法测酶活性的变异系数分别为0.84、2.03,而进样量后者是前者的2.6倍,稀释倍数是前者的6倍,邻苯三酚自氧化法的精确度高于NBT光还原法,但灵敏度远不及后者。在测定两种植物SOD时,邻苯三酚自氧化法测得酶活性变异系数是NBT光还原反应测得酶活性变异系数的1.30~1.47倍,NBT光还原法具有稳定性、重现性好的特点,优于邻苯三酚自氧化法。结论 NBT光还原法是测定植物SOD及SOD标准品酶活的理想检测方法。

The comparison of two methods of testing superoxide dismutase activity

Objective To compare the pyrogallol autoxidation and nitroblue tetrazolium (NBT) photoreduction methods for determination of superoxide dismutase (SOD) enzyme activity. Methods The paper based on the different methods to determine the SOD activity of standard, alfalfa and tomato, respectively, also compare the accuracy and sensitivity. Results The results showed that pyrogallol autoxidation and NBT photoreduction method of enzyme activity coefficient of variation were 0.84, 2.03, and the sample quantity which was 2.6 times of the former, dilution ratio was 6 times of the former, and the accuracy of pyrogallol autoxidation was higher than NBT photoreduction method but the sensitivity was poor, in detecting the SOD activity of standard. However, Pyrogallol autoxidation activity coefficient of variation was measured by NBT photoreduction reaction between 1.30 to 1.47 times the activities was measured by the coefficient of variation, the NBT photoreduction method has preferable stability and reproducibility than pyrogallol autoxidation in determining the SOD activity. Conclusion NBT photoreduction is an ideal testing method to determine the SOD activity of standard and plants.