肝移植并免疫抑制剂联合用药后人血清差异蛋白质的鉴定

建立了基于质谱技术的应用免疫抑制剂肝移植后人血清中差异表达蛋白质的分析方法。肝移植前后的人血清用双向凝胶电泳分离，硝酸银染色，选取的差异蛋白质斑点用表面活性剂Rapigest SF变性和胰蛋白酶进行胶内酶解后，采用纳升超高效液相色谱 电喷雾串联质谱（nanoUPLC-ESI-MS/MS）进行鉴定。本实验明确鉴定了9个显著差异表达的蛋白质斑点，对应8个不同的蛋白质，所发现的差异蛋白质多与免疫调节和肝病有关，可为深入研究肝移植术后免疫抑制剂抗排斥作用的分子机理提供线索。

Identification of the Differentially Expressed Proteins in Human Serum after Liver Transplantation and Immunosuppressant Combination Therapy

The mass spectrometry method was established for analysis of differentially expressed protein in human serum after liver transplantation with immunosuppressive agent. The proteins in serum before and after liver transplantation were separated by two-dimensional gel electrophoresis with silver staining, the differentially expressed proteins on the gel were digested with surfactant Rapigest SF and trypsin and then identified by nanoUPLC-ESI-MS/MS. In the experiment, 9 differentially expressed protein spots were identified clearly, corresponding to 8 different proteins. It was found that differentially expressed proteins are mostly related to immune regulation and liver disease, and it may provide clues for the in-depth study the anti-rejection effect of immunosuppressive agent after liver transplantation.