Dynamics of [Ca2+] in the endoplasmic reticulum and cytoplasm of intact HeLa cells. A comparative study |
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Authors: | MJ Barrero M Montero J Alvarez |
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Affiliation: | Departamento de Bioquímica y Biología Molecular y Fisiología, Instituto de Biologia y Genética Molecular, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Científicas, E-47005 Valladolid, Spain. |
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Abstract: | We have measured the Ca2+] in the endoplasmic reticulum (Ca2+]er) of intact HeLa cells at both 22 degrees C and 37 degrees C using endoplamsic reticulum-targeted, low Ca2+ affinity aequorin reconstituted with coelenterazine n. Aequorin consumption was much slower at 22 degrees C, and this allowed performing a much longer study of the dynamics of Ca2+]er. The steady-state Ca2+]er (500-600 microM) was not modified by the temperature, although both the rates of pumping and leak were decreased at 22 degrees C. The behavior of both Ca2+]er and cytoplasmic Ca2+] (Ca2+]c) after the addition of increasing concentrations of agonists and/or Ca2+-ATPase inhibitors, or following incubation in Ca2+-free medium were compared. We show that agonists induce a fast but relatively small decrease in Ca2+]er, which is enough to produce a sharp increase in Ca2+]c. Termination of Ca2+ release is controlled by feedback inhibition of the inositol 1,4,5-trisphosphate receptors by Ca2+]c, a mechanism that appears to be designed to release the minimum amount of Ca2+ necessary to produced the required Ca2+]c signal. We also show that Ca2+ release is inhibited progressively when Ca2+]er decreases below a threshold of about 150 microM, even in the absence of Ca2+ pumping or -Ca2+-c increase. This effect is consistent with a regulation of the inositol 1,4,5-trisphosphate-gated channels by Ca2+]er. |
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