| 苜蓿叶蛋白抗氧化肽水解用酶的筛选研究 |
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| 引用本文： | 谢正军,金征宇.苜蓿叶蛋白抗氧化肽水解用酶的筛选研究[J].食品科学,2007,28(7):342-346. |
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| 作者姓名： | 谢正军 金征宇 |
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| 作者单位： | 江南大学食品学院,江苏,无锡,214122 |
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| 摘 要： | 研究了中性蛋白酶AS1.398、Neutrase及碱性蛋白酶Alcalase FG2.4L、Proleather FG-F、Protease S水解苜蓿叶蛋白的进程特性,考察了五种蛋白酶水解物及不同DH的Alcalase FG2.4L酶解物对二苯代苦味肼基自由基（DPPH·）清除能力的影响。结果表明,碱性蛋白酶Alcalase FG2.4L是制备苜蓿叶蛋白抗氧化肽的最适水解酶,其DH20的酶解物浓度为1.6mg/ml时对DPPH·清除率达80%以上;其酶解物中相对分子质量小于1000的组分所占比例最高,达到67.86%;酶解物的DH与DPPH·清除率之间不存在线性关系。
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| 关 键 词： | 苜蓿叶蛋白 抗氧化肽 蛋白酶 二苯代苦味肼基自由基(DPPH·) |
| 文章编号： | 1002-6630（2007）07-0342-05 |
| 修稿时间： | 2007-05-15 |
Screen of Protease for Preparing Antioxidation Peptides from Alfalfa Leaf Protein |
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| XIE Zheng-jun,JIN Zheng-yu.Screen of Protease for Preparing Antioxidation Peptides from Alfalfa Leaf Protein[J].Food Science,2007,28(7):342-346. |
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| Authors: | XIE Zheng-jun JIN Zheng-yu |
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| Affiliation: | School of Food Science and Technology, Jiangnan University, Wuxi 214036, China |
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| Abstract: | The best protcasc was chosen according to the antioxidation effect of hydrolysates, which were prepared by hydrolyzing for alfalfa leaf protein with AS 1.398, Ncutrasc, Alcalasc, Proleather FG-F and Protease S respectively, against DPPH radical. The results showed that the hydrolysatcs prepared by Alcalase FG 2.4L has the strongest antioxidation against DPPH radical. |
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| Keywords: | alfalfa leaf protein antioxidation peptides protease DPPH radical |
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