| 烟草体细胞杂交优质抗病株系的选育 |
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| 引用本文： | 孙学永,林国平,殷凤生,杨其光,周应兵,杨华应.烟草体细胞杂交优质抗病株系的选育[J].烟草科技,2003(7):36-40. |
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| 作者姓名： | 孙学永 林国平 殷凤生 杨其光 周应兵 杨华应 |
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| 作者单位： | 1. 安徽省农科院烟草研究所,安徽省凤阳县洪武路63号,233100
2. 安徽农业大学生物工程系,合肥市长江西路130号,230036 |
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| 基金项目： | 安徽省科委“烟草细胞工程和基因工程优质抗病育种”课题资助 |
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| 摘 要： | 为了选育抗花叶病的烟草新品种 ,用G 140与抗花叶病的野生波叶烟草 (N .undulata)进行了体细胞杂交 (即两种烟草的体细胞原生质体融合 ) ,其后代经过形态学鉴定 ,过氧化物酶同功酶酶谱鉴定、细胞学染色体数目检测 ,确定为体细胞杂种植株。采用定向筛选和回交手段选育体细胞杂种植株 ,经过 3年的田间筛选鉴定 ,得到了体细胞杂交单株自交系 ,从中再经筛选和攻毒试验获得了几个综合农艺性状好 ,抗或耐花叶病的优良单株和株系
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| 关 键 词： | 烟草 体细胞杂交 抗病株系 选育 |
| 文章编号： | 1002-0861(2003)07-0036-05 |
| 修稿时间： | 2003年2月16日 |
Breeding of Quality and Mosaic Resistant Tobacco by Somatic Cell Hybrid |
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| SUN XUE-YONG.Breeding of Quality and Mosaic Resistant Tobacco by Somatic Cell Hybrid[J].Tobacco Science & Technology,2003(7):36-40. |
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| Authors: | SUN XUE-YONG |
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| Abstract: | For breeding new TMV-resistant tobacco varieties, the interspecific somatic cell hybrid between N. tobacum CV.G 140 and N. undulata was conducted. The TMV-resistant progeny were selected and identified by morphological differences, peroxidase labeling, and chromosome numbers, from which the plants of somatic cell hybrid were obtained. Several quality and virus-resistant variants were obtained by selecting and identifying during three growth seasons in field experiments and virus inoculation experiments. |
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| Keywords: | Tobacco Progeny of somatic cell hybrid Resistant variants Breeding |
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