| 酸性磷酸酶在大鼠脉络丛上皮细胞内的亚细胞定位 |
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| 引用本文： | 王维平,刘建华,吴东霞,郭艳苏,杨程,吴淑玉,刘惠苗,吴丽玲.酸性磷酸酶在大鼠脉络丛上皮细胞内的亚细胞定位[J].电子显微学报,2008,27(2):131-135. |
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| 作者姓名： | 王维平 刘建华 吴东霞 郭艳苏 杨程 吴淑玉 刘惠苗 吴丽玲 |
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| 作者单位： | 1. 河北医科大学第二医院神经内科,河北,石家庄,050000;河北省心脑血管病研究所,河北,石家庄,050000
2. 河北医科大学第二医院神经内科 |
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| 摘 要： | 研究大鼠脑室脉络丛上皮细胞内酸性磷酸酶(Acid phosphatase,AcPase)的超微结构分布.取成年SD大鼠侧脑室、第三脑室和第四脑室的脉络丛组织,按硝酸铅法进行酸性磷酸酶电镜酶细胞化学染色显示.透射电镜下ACPase阳性反应物仅见于脉络丛上皮细胞内的初级和次级溶酶体上.通过延长孵育时间和改变固定条件(戊二醛浓度为0.5%)在脉络丛上皮细胞内的高尔基复合体扁囊和囊泡中也观察到了ACPase阳性反应物.各脑室脉络丛上皮细胞酸性磷酸酶的分布未见明显差异.文中讨论了脉络丛上皮细胞内ACPase的分布特征及其功能.
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| 关 键 词： | 酸性磷酸酶 脉络丛上皮 大鼠 电镜 细胞化学 酸性磷酸酶 大鼠 脉络丛 上皮细胞 胞内 亚细胞定位 choroid epithelial cell acid localization 功能 分布特征 差异 囊泡 复合体 高尔基 浓度 戊二醛 条件 固定 |
| 文章编号： | 1000-6281(2008)02-0131-05 |
| 修稿时间： | 2007年6月21日 |
Subcellular localization of acid phoshatase in the epithelial cell of choroid plexus in rat |
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| WANG Wei-ping,LIU Jian-hua,WU Dong-xia,GUO Yan-su,YANG Cheng,WO Shu-yu,LIU Hui-miao,WU Li-ling.Subcellular localization of acid phoshatase in the epithelial cell of choroid plexus in rat[J].Journal of Chinese Electron Microscopy Society,2008,27(2):131-135. |
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| Authors: | WANG Wei-ping LIU Jian-hua WU Dong-xia GUO Yan-su YANG Cheng WO Shu-yu LIU Hui-miao WU Li-ling |
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| Affiliation: | WANG Wei-ping1,2,LIU Jian-hua1,WU Dong-xia1,GUO Yan-su1,YANG Cheng1,WU Shu-yu1,LIU Hui-miao1,WU Li-ling1(1.Department of Neurology,The Second Hospital of Hebei Medical University,2.Institute of Cardiocerebrovascular Disease,Shijiazhuang Hebei 050000,China) |
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| Abstract: | The activities of the acid phosphatase of choriod plexus in rat was observed by electron microscopic cytochemistry.Choroid plexus of the three different ventricles of adult rats were removed and incubated following the lead nitrate method.Under the transmission electron microscope,the electron dense reaction products were only noticed at the site of lysosomes in the specimens fixed with 2% paraformaldehyde and 2%glutaraldehyde,the positive reaction products were noticed in the cisternal and vesicles of Golg... |
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| Keywords: | acid phosphatase choroid plexus epithelium rat electron microscope cytochemistry |
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