藠头提取物对氟尿嘧啶的增效减毒作用的研究

研究了藠头提取物对人宫颈癌Hela细胞和人胃癌BGC-823细胞体外生长的影响、该提取物与5-FU对抑制这两种癌细胞中的协同作用,探讨了该提取物对5-FU所致小鼠免疫功能抑制的保护作用,并测定了药物对两种癌细胞增殖抑制率的影响和检测药物对小鼠腹腔巨噬细胞吞噬功能及淋巴细胞活性的影响。发现药物作用48h后,联合用药对胃癌细胞的抑制率高于单药组,并表现明显的协同作用;联合用药且能明显拮抗5-FU所致体重降低、腹腔巨噬细胞功能降低及淋巴细胞活性降低等毒副作用。表明藠头提取物与5-FU联合使用能有明显提高5-FU抗肿瘤及降低其毒副作用。

Role of Bioactive Components of Allium chinense in Increasing Efficacy and Decreasing Toxicity of Fluorouracil

The present study was to investigate the inhibition of the bioactive components of Allium chinense to cervical cancer Hela cells and human gastric cancer cell line BGC-823 in vitro, its effect on the sensitivity of Hela and BGC-823 cells to 5-FU, and its protection for the immunological function of mice against the toxicity of 5-FU. The inhibition rate of cell proliferation was assayed by MTT method and the effect of the bioactive components of Allium chinense combined with 5-FU on mice was tested by the phagocytosing functions of macrophagocytes in abdominal cavity and activity of lymphocyte. The results showed that the bioactive components of Allium chinense combined with 5-FU markedly inhibited the proliferation of Hela cells and BGC-823 cell and decresed toxicity and side-effect of 5-FU to macrophagocyte and lymphocyte. It is suggested that the bioactive components of Allium chinense combined with 5-FU have an obvious effect on inhibiting tumor with decreased toxicity and side-effect.