Development and Validation of a HPLC Method for Determination of Flavone Glycoside-Camellianin B in Cephalotaxus Sinensis

A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis (C.sinensis) a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid chromatography.Chromatographic separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min-1 with the acetonitrilewater containing 0.1％ formic acid (19:81,v/v).The detection wavelength was set at 330 nm.The calibration curve was linear over the concentration range of 0.25-50 μg·mL-1with correlation coefficients larger than 0.999 5.The limit of detection and limit of quantification were 0.09 μg·mL-1and 0.25 μg·mL-1,respectively.The precisions and accuracy for all samples were acceptable.The validated method has been successfully applied for the quantification of camellianin B in C.sinensis harvested in different months and may also be used as the quality evaluation of this herb medicine.