小红豆抗菌蛋白的分离纯化及其功能活性

研究旨在从小红豆中分离纯化得到一种具有抗菌活性的蛋白,对其部分生物活性进行表征和研究。通过缓冲液抽提（NaAc-HAc,20 mmol/L,pH5.4）、硫酸铵分级沉降、Affi-gel blue gel亲和色谱、Sephadex SP C-25离子交换色谱及Sephadex G-50凝胶过滤色谱进行分离纯化,经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析鉴定其相对分子质量,并对其抗真菌活性进行表征。结果表明,从小红豆中分离纯化出的一种抗菌蛋白,经SDS-PAGE鉴定达到电泳纯,且其相对分子质量约为4 kDa,通过纯化的抗菌蛋白对来自茄子、甜瓜及苦瓜的尖孢镰刀菌的抑制实验,证明此抗菌蛋白具有较强的抗真菌活性。从小红豆中分离纯化出的一种分子质量约为4 kDa的抗菌蛋白,其对真菌具有抑制作用,且其抑制率对浓度的依赖性较高。 

Isolation and purification of an antifungal protein from small red bean and its functional properties

This essay was aim to isolate, purificate and partial characterize the proteins with antifungal activity from the small red beans. A protein with antifungal activity was isolated and purified from small red beans by the procedure containing extraction, ammonium sulfate precipitation, Affi-gel blue gel chromatography, ion-exchange chromatography, and gel filtration chromatography.SDS-PAGE was used to analysis its purity and molecular weight.The result showed that the antifungal protein isolated and purified from small red beans exhibited a molecular mass of 4 kDa in SDS-polyacrylamide gel electrophoresis.Antifungal activity experiment showed that the protein had a strong inhibitory activity towards the growth of Fusarium oxysporum from eggplant, muskmelon and bitter gourd.An antifungal protein with a molecular mass of 4 kDa was isolated from small red beans.It exerted inhibitory activity on Fusarium oxysporum and had a high dependence on the concentration of protein.