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超高效液相色谱-串联质谱法测定哈密瓜和茄子中氟啶虫胺腈的残留量
引用本文:陈爱松, 崔银仓, 羌松, 张强, 周成松, 刘淑华, 靳改龙, 张以和, 马德英. 超高效液相色谱-串联质谱法测定哈密瓜和茄子中氟啶虫胺腈的残留量[J]. 食品工业科技, 2017, (20): 260-265. DOI: 10.13386/j.issn1002-0306.2017.20.046
作者姓名:陈爱松  崔银仓  羌松  张强  周成松  刘淑华  靳改龙  张以和  马德英
作者单位:1.1. 新疆农业大学农学院农林有害生物监测与安全防控重点实验室
摘    要:为满足农产品与食品安全检测的需求,本文验证GB/T 20769-2008对哈密瓜和茄子样品中氟啶虫胺腈残留量测定的适用性并简化前处理步骤。果蔬样品用乙腈为提取剂,采用组织捣碎、固液提取法提取果蔬中的氟啶虫胺腈,通过盐析离心,氨基柱固相萃取净化,甲醇∶二氯甲烷(5∶95,v∶v)洗脱样品中的氟啶虫胺腈,甲醇∶水(1∶1,v∶v)定容,过0.2μm滤膜;在UPLC-MS/MS负离子扫描多反应监测模式下进行分析。氟啶虫胺腈在哈密瓜与茄子两种基质中的线性相关系数r分别为0.9998和0.9992。在500、200、50μg/L三个添加水平下的回收率为81.00%107.76%,RSD在2.56%3.82%范围内,方法检出限(LOD)在哈密瓜与茄子中分别为0.72μg/kg与0.62μg/kg。该方法的准确度和精密度均符合残留分析的要求,而且高效、快速、简单、灵敏度高、确认性和实用性强,适用于氟啶虫胺腈在哈密瓜、茄子残留检测的常规方法。 

关 键 词:氟啶虫胺腈  超高效液相色谱-串联质谱法  茄子  哈密瓜  残留量
收稿时间:2017-04-21

Determination of sulfoxaflor residues in melon and eggplant by ultra-high performance liquid chromatography-tandem mass spectrometry
CHEN Ai-song, CUI Yin-cang, QIANG Song, ZHANG Qiang, ZHOU Cheng-song, LIU Shu-hua, JIN Gai-long, ZHANG Yi-he, MA De-ying. Determination of sulfoxaflor residues in melon and eggplant by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2017, (20): 260-265. DOI: 10.13386/j.issn1002-0306.2017.20.046
Authors:CHEN Ai-song  CUI Yin-cang  QIANG Song  ZHANG Qiang  ZHOU Cheng-song  LIU Shu-hua  JIN Gai-long  ZHANG Yi-he  MA De-ying
Affiliation:1.College of Agronomy, Xinjiang Agricultural University, Key Laboratory of the Pest Monitoring and Safety Control on Crop and Forest at Universities of Xinjiang Uyghur Autonomous Region
Abstract:It is verified that GB/T 20769-2008 is also suitable for the determination of the residue of sulfoxaflor in the samples of cantaloupe and eggplant and simplied the pretreatment steps. Samples of melon and eggplant were initially extracted with acetonitrile, salting out by Na Cl, cleaned up by aminopropyl ( NH2) cartridge, eluted by methanol and dichloromethane ( 5∶ 95, v∶ v) , metered by methanol and water ( 1 ∶ 1, v ∶ v) . After filtered with a 0.2 μm membrane, the resulting samples determined by UPLC-MS/MS performing on negative electrospray ionization and the multiple reactions monitoring mode ( MRM) .The matrix-matched external standard calibration curves were used for quantitative analysis.The linearly dependent coefficient r of sulfoxaflor for cantaloupe and eggplant were 0.9998 and 0.9992, respectively.The added recoveries were between81.00% 107.76% in the concentration range of 50 ~ 500 μg/L. The lowest limit of detection ( LOD) was 0.72 μg/kg and0.62 μg/kg, and the relative standard deviations ( RSD, n = 6) were in the range of 2.56% ~ 3.82%. The method can meet the requirements for determination of sulfoxaflor residue in cantaloupe and eggplant, and it is a quick, easy, effective, sensitive and accurate method.
Keywords:sulfoxaflor  ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)  cantaloupe  eggplant  residue
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