SSR荧光标记技术分析东四省典型绿豆品种的遗传多样性

东北是我国绿豆主产区之一,品种资源相对丰富。分析不同绿豆品种间遗传背景差异,明确其群体遗传结构,有助于提高品种资源在育种中的利用效率。本研究利用8对SSR引物对东四省40份绿豆品种进行荧光标记毛细管检测分析,共检测出43个等位变异,平均为5.4个,多态信息含量(PIC)变幅介于0.40~0.75之间,平均为0.55。比较分析发现,平均等位变异数目的分布为:黑龙江省(3.63)=辽宁省(3.63)>内蒙古自治区(3.50)>吉林省(2.88);多态信息含量(PIC)的分布为:黑龙江省(0.530)>辽宁省(0.527)>内蒙古自治区(0.469)>吉林省(0.382),即黑龙江省遗传变异水平最高,吉林省最低。聚类分析表明,黑龙江和辽宁的绿豆种质为组群Ⅰ,内蒙古和吉林为组群Ⅱ。本研究初步明确了东北各省(区)绿豆品种的遗传多样性水平,为资源的再收集及育种利用提供了参考,并为绿豆资源的深入利用奠定了基础。 

Genetic diversity of typical mungbean varieties in Northeast China by fluorescent SSR markers

Northeast China is one of the main producing areas of mung bean with abundant resource. It helps to improve the utilization efficiency of breeding that clear the genetic background differences between different mung bean varieties. Capillary electrophoresis detection with fluorescent ssr markers were used to analyze 40 typical mung bean varieties by using 8 SSR molecular markers.A total of 43 alleles were identified with an average of 5.4 alleles per locus. The polymorphic information content ( PIC) of tested materials detected by different primers varied from 0.40 to 0.75 with an average of 0.55.The distribution of the average number of allelic variation was Heilongjiang ( 3.63) = Liaoning ( 3.63) > Neimenggu ( 3.50) > Jilin ( 2.88) .The distribution of polymorphic information content ( PIC) was Heilongjiang ( 0.530) > Liaoning ( 0.527) > Neimenggu ( 0.469) >Jilin ( 0.382) . The analysis of genetic diversity of mungbean resources from 4 geographic origin indicated that Heilongjiang province was the most diversity region, and the last was Jilin province.Cluster analysis showed that germplasm from Heilongjiang province and Liaoning province were the first group, and those from Inner Mongolia and Jilin province were the second group.The genetic diversity of mung bean varieties in Northeast China was preliminarily identified in this study which provided reference for the recollection and breeding utilization of resources and laid the foundation for the further utilization of mung bean resources.