核桃肽NP-1对大鼠酒精性情绪及认知损伤的改善效应

为评价核桃肽NP-1对SD大鼠酒精性情绪及认知损伤的改善效应,将40只SPF级SD大鼠随机分为空白对照组、酒精模型组、NP-1低、中、高剂量组,每组8只。采用10%酒精作为唯一水源,连续18周构建大鼠情绪及认知损伤模型组,同时空白对照组每天给予蒸馏水,低、中、高剂量组饮水中分别添加0.32、0.96、2.88 mg/m L的NP-1。每天测定大鼠饮水量变化,定期观察NP-1对大鼠体重的影响,高架十字迷宫检测大鼠情绪焦虑水平,强迫游泳实验检测情绪抑郁水平,Morris水迷宫检测空间学习记忆能力,血细胞自动分析仪进行血常规免疫相关指标分析。结果表明,与空白对照组相比,模型组大鼠高架十字迷宫中开放臂时间比例（OT%）、强迫游泳时累计不动时间、水迷宫潜伏期、平台穿越次数和目标象限探索时间、白细胞和淋巴细胞水平均显著改变（p<0.05）。与模型组相比,不同剂量NP-1组高架十字迷宫OT%、水迷宫实验平台潜伏期均显著降低（p<0.05）,高剂量组平台位置穿越次数和目标象限探索时间显著增加（p<0.05）,白细胞和淋巴细胞水平显著降低（p<0.05）,其中高剂量组改善效果最明显,提示NP-1能有效改善酒精导致的情绪及认知损伤。 

The ameliorative effects of walnut peptide NP-1 on the alcoholic emotions and cognitive impairments in rats

In order to evaluate the ameliorative effects of walnut peptide NP-1 on the alcoholic emotions and cognitive impairments in SD rats, totally 40 healthy SD rats were averagely divided into control group, alcohol model group, NP-1 low, medium and high dose group, each group for eight. Using 10% alcohol as the only water source, emotion and cognitive impairment model were established. At the same time, the control group was given distilled water, low, middle and high dose groups were given different concentrations of NP-1 ( 0.32, 0.96, 2.88 mg/m L) .To determine the change of drinking quantity in rats, observe the effect of NP-1 on the change of weight in rats, elevated plus maze tested anxiety, morris water maze tested spatial learning and memory ability, forced swimming tested detection of depression. Using the automatic blood cell analyzer analyzed routine blood indexes. Compared with the control group, alcohol model group of open arm time in the elevated plus maze, immobility time on the forced swimming test and latent period of Morris water maze prolonging increased significantly ( p < 0.05) . The times of passing through the platform position and ratio of swimming distance on the target quadrant decreased, the ratio of leukocyte and lymphocyte increased.Compared with alcohol model group, the open arm time of different dose NP-1 in the elevated plus maze, the immobility time on the forced swimming test and latent period of Morris water maze reduced ( p < 0.05) . The times of passing through the platform position and ratio of swimming distance on the target quadrant of high dose group increased, elevated of the number of leukocyte and lymphocytes reduced abnormally. The effect of high dose group was the most significant, suggesting that NP-1 can effectively improve the emotional and cognitive impairment caused by alcohol.