卡拉胶酶的发酵培养基及培养条件优化

以生物量与卡拉胶酶活为指标,研究发酵培养基组成和培养条件对菌株ASY5产卡拉胶酶的影响,优化菌株Pseudoalteromonas carrageenovora ASY5产卡拉胶酶的培养基和培养条件,以提高卡拉胶酶的产量。结果表明,最优培养基和培养条件为:卡拉胶5 g/L,胰蛋白胨5 g/L,Na Cl 20 g/L,Ca Cl₂0.2 g/L,Na H₂PO₄·2H₂O 1.3 g/L,Na₂HPO₄·12H₂O3.8 g/L,温度18℃,初始p H为6.5,接种量2%,装液量70 m L。在优化工艺条件下,菌株ASY5的生物量和卡拉胶酶活比初始条件分别提高了80.4%和52.2%,菌株ASY5发酵产卡拉胶酶的能力大幅度提高。 

Optimization of medium composition and culture conditions for carrageenase production

Biomass and carrageenase activity was used as the index, the effects of medium compositon and culture conditons on cell growth and enzyme production was investigated in shaking culture of P.carrageenovora ASY5.In order to increase the yield of carrageenase, the medium compositon and culture conditons were optimized for fermentation of Pseudoalteromonas carrageenovora ASY5.The optimal medium compostion was composed of carrageenan 5 g/L, tryptone 5 g/L, Na Cl 20 g/L, Ca Cl₂0.2 g/L, Na H₂PO₄·2H₂O 1.3 g/L, Na₂HPO₄·12H₂O 3.8 g/L, while the optimal culture conditions were determined as tempreture 18℃, initial p H6.5, inculation volume 2%, liquid volume 70 m L.Under the optimum fermentation process, the production capability of carrageenase by P.carrageenovora ASY5 was significantly improved.Compared to the basic technical contidtions, the biomass and enzyme activity at the optimum technique were increased by 80.4%and 52.2%, respectively.