两步酶法提取裂壶藻油的工艺优化

采用两步酶法提取裂壶藻油并对工艺进行优化。通过单因素和正交实验,确定了提取裂壶藻油的最佳工艺条件,并通过验证实验对正交结果进行验证。得到的最佳工艺条件为:料液比1∶7,中性蛋白酶添加量9%,酶解温度45℃,酶解时间3 h,酶解pH6.5;碱性蛋白酶的添加量为12%,酶解温度70℃,酶解时间6 h,酶解pH9.5。在此条件下的清油得率可以达到90.22%。气相色谱-质谱（GC-MS）分析裂壶藻油中不饱和脂肪酸含量44.13%,DHA含量为32.06%。 

Optimization of two-step enzymatic extraction of oil from Schizochytrium sp.

Two-step enzymatic hydrolysis was uesd to extract oil from Schizochytrium sp. and the extraction conditions were optimized.The optimal extraction processing of oil from Schizochytrium sp were explored by single factor and orthogonal experiments, and verification experiments was used to verify the result of orthogonal experiments. The optimal extraction conditions was obtained as follows: liquid/solid ratio 1 ∶ 7, neutral protease addition 9%, enzymatic hydrolysis temperature45 ℃, enzymatic hydrolysis time 3 h, enzymatic hydrolysis pH6.5, alkaline protease addition 12%, enzymatic hydrolysis temperature 70 ℃, enzymatic hydrolysis time 6 h, enzymatic hydrolysis pH9.5. Under these conditions, the clear oil extraction ratio from Schizochytrium sp. reached 90.22%. Gas chromatography-mass spectrometry ( GC-MS) analysis showed that thecontent of unsaturated fatty acids was 44.13% and the DHA content was 32.06%.