高效液相色谱-二极管阵列联用技术同时检测苹果醋和甘蔗醋中11种酚类物质

利用高效液相色谱联合二极管阵列技术（HPLC-DAD）建立一种可以同时检测不同苹果醋和甘蔗醋中11种酚类物质的分析方法。使用OMNI Hubble C₁₈色谱柱（250 mm×4.6 mm,5μm）,流动相A为甲醇-甲酸（100∶2,v/v）,B为10 mmol/L磷酸氢二钾-磷酸水溶液（200∶1,v/v）,流速为1.0 m L/min,进样量为20μL,梯度洗脱,柱温为35℃,二极管阵列扫描波长范围210⁴00 nm。应用本方法可以检测出不同苹果醋和甘蔗果醋中香草醛、香豆素、绿原酸、咖啡酸、阿魏酸、对香豆酸、芹菜素、木犀草素、肉桂酸、山奈酚、芒柄花黄素等11种酚类物质,其中甘蔗醋中富含绿原酸等10种酚类物质成分。本方法回收率在84.1%¹18.2%之间,结果准确可靠,简单易行,适合用于各类果醋中酚类物质的分析检测。 

Simultaneous determination of 11 phenolic compounds in apple vinegar and sugarcane vinegar by HPLC-DAD

A new method for simultaneous determination of 11 phenolic compounds in fruit vinegar, such as different kinds of apple vinegar and sugarcane vinegar, by high performance liquid chromatography-diode array detector (HPLC-DAD) was established in this study.The phenolic compounds were separated by OMNI BOND with Hubble C₁₈column (250 mm×4.6 mm, 5μm) at 35℃.The mobile phase A was Me OH-HCOOH solution (100∶2, v/v) and the mobile phase B was 10 mmol/L K₂HPO₄-H₃PO₄ (200∶1, v/v) , eluted at a flow rate of 1.0 m L/min by a gradient elution program.The injection volume was20μL and the eluted components were monitored at 210⁴00 nm.Under the optimized condition, 11 phenolic compounds in different kinds of apple vinegar and sugarcane vinegar were separated and simultaneous determined, including coumarin, vanillin, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, apigenin, luteolin, cinnamic acid, kaempferol and formononetin, in which 10 phenolic components were found and determined in sugarcane vinegar.The recovery of the above method was 84.1%¹18.2%.The method is accurate, reliable, simple, feasible and suitable for determination of phenolic compounds in fruit vinegar.