响应面法分析多酚物质间的协同抗氧化作用

以DPPH自由基清除率为抗氧化活性评价指标,选择抗氧化较强的茶多酚、儿茶素、绿原酸、没食子酸为影响因素,运用响应面法分析多酚物质间的抗氧化协同作用。实验结果表明,茶多酚、儿茶素、绿原酸、没食子酸对DPPH自由基清除率的影响非常显著,影响顺序依次为没食子酸、儿茶素、茶多酚、绿原酸。多酚物质间存在一定的交互作用,但交互作用不显著(除茶多酚与没食子酸)。响应面实验中除了1、13组外,其他复配组合的协同系数SE均小于1,表明多酚物质间的协同抗氧化作用不明显。 

Synergistic antioxidant activity among polyphenols by response surface methodology

DPPH free radical clearance was used as the evaluation index of antioxidant activity. Because tea polyphenols, catechins, chlorogenic acid and gallic acid had stronger antioxidant, they were selected as influencing factors. The synergistic antioxidant activity of the mixture of polyphenols was analyzed by the response surface methodology.Tea polyphenols, catechins, chlorogenic acid, gallic acid had significant effect on the capacity of scavenging DPPH free radicals.The capacity of scavenging DPPH free radicals was as follows: gallic acid, catechin, tea polyphenols, chlorogenic acid. There were interactions between polyphenols, but the interaction was not significant for the capacity of scavenging DPPH free radicals ( except gallic acid and tea polyphenols) . Except the first experiment and the thirteen experiment, the collaborative SE of other response surface experiments were less than 1, and the synergy of polyphenols was not obvious.