碱法预处理琼枝麒麟菜提取卡拉胶的工艺优化

研究碱法预处理琼枝麒麟菜提取卡拉胶的工艺,通过响应面法优化工艺条件。在单因素实验的基础上,以KOH碱液质量分数、碱预处理温度和碱预处理时间为影响因素,以卡拉胶的产率和凝胶强度为响应值建立二次回归方程,通过响应面分析得到碱预处理琼枝麒麟菜提取卡拉胶的最佳工艺。实验结果显示最佳碱法预处理工艺为KOH碱液质量分数10%、碱预处理温度56℃和碱预处理时间6 h,在此条件下,卡拉胶产率75.23%±2.16%、凝胶强度(1378.5±16.27)g/cm~2。优化后的工艺有效改善了琼枝麒麟菜提取卡拉胶的生产条件。 

Technological optimization of alkali pretreatment in the carrageenan extraction from Eucheuma gelatinae

The technological optimization of alkali pretreatment in the course of carrageenan extraction from Eucheuma gelatinae was studied by the response surface methodology. On the basis of single-factor investigation, the quadratic regression equations about yield and gel strength ( GS) of carrageenan were established regarding KOH mass fraction, alkali-pretreating temperature and alkali-pretreating time as influence factors, and yield and strength of carrageenan as response values, the optimal conditions for the alkali pretreatment were analyzed by the response surface methodology.The optimizing process parameters were as follows:KOH mass fraction 10%, alkali pretreating temperature 56 ℃ and alkali pretreating time 6 h, under the conditions, yield and GS of carrageenan from Eucheuma gelatinum were 75.23% ± 2.16% and ( 1378.5 ± 16.27) g/cm2 respectively.The optimized process could effectively improve the producting conditions for the carrageenan extraction from Eucheuma gelatinae.