响应面法优化灵芝孢子粉中灵芝酸的破壁提取工艺

目的:以超声提取法为主,漆酶破壁法为辅助提取灵芝孢子粉中的灵芝酸,对影响提取得率的几个因素进行优化。方法:借助Design-Expert软件,利用Placket-Burman实验从超声时间、超声次数、溶剂、料液比、酶量、酶解时间、酶解温度和p H这8个影响提取得率的因素中筛选出四个主要因素,分别为超声时间、超声次数、溶剂和酶解时间,在此基础上,用Box-Behnken实验设计及响应面分析法进行回归分析。结果:当超声时间为3.2 h,溶剂用97%的乙醇,超声次数2次,酶解时间1.2 h时,提取得率达到最高,为3.40%,与初始提取得率2.4%相比,提取得率提高了1.4倍。结论:采用漆酶破壁与超声提取方法较大幅度提高灵芝酸的提取得率,并缩短了提取时间。 

Optimization of wall-breaking extraction technology of Ganoderic acid from Ganoderma Spores ( GS) using response surface methodology

Objective: Ganoderma Spores (GS) was used as raw material as the ultrasonic extraction as the main method and laccase wall-breaking method the secondary one to extract Ganoderic acid, the extraction factors affected the extraction ratio were optimized.Methods: Ultrasonic time, ultrasonic frequency, solvent and enzymolysis time were identified as the main factors from asonic time, ultrasonic frequency, solvent, solid-liquid ratio, enzyme amount, enzymolysis time, digestion temperature and p H which influenced the extraction ratio using experimental design of Placket-Burman (PB) by design-expert software.On this basis, the experimental design of Box-Behnken (BBD) and response surface analysis method was used for regression analysis.Results: When the ultrasonic time was 3.2 h, the solvent was 97% ethanol, ultrasonic frequency of 2 times, enzymolysis time for1.2 h, reached the highest extraction rate was 3.40%, and the extraction yield increased 1.4 times compared with the initial design.The extraction rate of ganoderma lucidum acid was increased greatly and the extraction time was shorten by the combined ues of cell wall disruption with lasscase and ultrasonic extraction.