大孔树脂AB-8分离纯化伦晚脐橙总黄酮

考察了不同大孔树脂对伦晚脐橙残次果总黄酮的吸附与解吸性能,筛选出适宜纯化的大孔树脂AB-8。以芸香柚皮苷、橙皮苷、香蜂草苷、甜橙黄酮、川陈皮素、橘皮素6种标准品绘制标准曲线,采用高效液相色谱法（HPLC）进行定性、定量分析。通过静态与动态吸附、解吸,得出大孔树脂AB-8纯化伦晚脐橙残次果总黄酮的最佳工艺条件为:0.75 mg/m L的p H=3上样液,上样体积为3.5倍柱体积（BV）,上样流速为2 BV/h;80%乙醇为洗脱剂,2 BV/h流速洗脱,洗脱体积为5 BV,解吸率达90.73%。经大孔树脂纯化后,提取物中总黄酮含量由纯化前36.04 mg/g增加到纯化后334.54 mg/g。HPLC分析结果表明,AB-8分离纯化伦晚脐橙总黄酮不会造成单体黄酮组成变化,其中主要黄酮类物质是芸香柚皮苷、橙皮苷,占总量的91.14%。该工艺能有效地富集伦晚脐橙黄酮类,去除糖、色素等物质,且对伦晚脐橙黄酮组分没有影响。 

Separation and purification of total flavonoids from lane late navel orange by AB-8 macroporous resins

The adsorption and desorption of total flavonoids from lane late navel orange fruits were investigated by using 3macroporous resins.Results indicated that AB-8 resin was appropriate to purify the total flavonoids.Then the polyphenols from orange fruits were qualitatively and quantitatively by HPLC ( high performance liquid chromatography) . To optimize the separation process, the static and dynamic of adsorption and desorption experiments were carried out, the optimal adsorption conditions were as follows: initial concentrations in sample solution 0.75 mg/m L, p H3.0, sample loading amount 3.5 BV, flow rate 2 BV/h.The optimal desorption parameters were 80% ethanol 5 BV, flow rate of 2 BV/h, the desorption ratio was 90.73%.After purification, the content of flavonoids in product was increased from 36.04 mg/g to 334.54 mg/g. HPLC analysis results found that the flavonoids profiles of lane late navel orange were not changed after adsorption and desorption by AB-8macroporous resin. The main flavonoids were narirutin and hesperidin, the percentage content to the total flavonoids was91.14%. The results showed that AB-8 resin revealed a good ability to enrichment total flavonoids from lane late navel orange, and the method can be referenced for the enrichment of total flavonoids from other material.