肉制品中6种人工合成色素的快速测定

目的:建立一种同时测定肉制品中柠檬黄、苋菜红、胭脂红、日落黄、诱惑红和酸性红6种人工合成色素的高效液相色谱法。方法:采用乙醇-氨溶液提取样品中的色素,经乙醚脱脂,用Agilent Poroshell 120 SB-C₁₈色谱柱分离,以乙腈-乙酸铵（0.02 mol/L）为流动相,二极管阵列检测器可变波长程序测定。结果:6种色素在浓度0¹0μg/mL均有良好的线性关系,线性系数均大于0.9999,加标回收率为70.33%⁹3.08%,检出限为0.12⁰.21 mg/kg,相对标准偏差均小于5%。结论:该方法操作简单、快捷,重现性好,灵敏度高,可同时测定肉制品中6种人工合成色素,适用于肉制品的快速检测。 

Rapid detection methods for six kinds of synthetic pigments in meat products

Objective: To establish a method for simultaneous determination of six kind of synthetic pigments including tartrazine, amaranth, carmine, sunset yellow FCF, allura red and carmosine in meat products by HPLC.Methods: Extracted with ethanol-ammonia-water solution in the samples, then defatted by ether.The pigments were separated on Agilent Poroshell 120 SB-C₁₈ column, by gradient elution with a mobile phases made up of 0.02 mol/L NH4 Ac and acetonitrile, determined by diode array detector ( DAD) .Results: Six kinds of synthetic pigments had good linear relations when the concentration was in the range of 0¹0 μg/mL and the average recoveries range of 70.33%⁹3.08%.The correlation coefficient of regression equations could be up to 0.9999 and relative standard deviation was less than 5%.Conclusion: The method was simple, quickly, sensitivity and good reproducibility, which could be applicable to determine six kinds of synthetic pigments in meat products for lots of samles superviser detection.