5株植物乳杆菌生物学特性及安全性评价

通过对不同分离源的5株植物乳杆菌进行产酸、抑菌、耐酸耐胆盐、耐药性、溶血性、产生物胺能力测定,结果显示:5株菌的产酸及抑菌能力较强,差异显著（p<0.05）;耐酸性能较强,2 h的存活率在85.67%⁹8.00%,PBIL1-002与其他各菌株之间差异显著（p<0.05）;耐胆盐能力相对较弱,2 h的存活率在21.17%⁵1.20%,除PBIL1-002与PBIL1-006外,其余各菌株间差异显著（p<0.05）;5株菌均对链霉素和万古霉素耐药,PBIL1-009和PBIL1-018对复方新诺明耐药;PBIL1-006和PBIL1-009对庆大霉素耐药;菌株不具溶血性能;定性检测菌株产生物胺能力显示,PBIL1-009代谢产生精胺,PBIL1-018代谢产生精胺和酪胺。说明不同分离源的菌株特性及安全性有一定差异,生产应用时需选择生物学特性佳且无安全隐患的菌株。 

Biological characteristics and safety evaluation of five Lactobacillus plantarum strains

In this paper, five Lactobacillus plantarum strains isolated from different sources were detected for acid production, bacteriostasis, acid resistance, bile salt resistance, antibiotic resistance, hemolysis and biological amine formation. The results showed that five strains exhibited the higher ability of acid production and bacteriostasis, with a significant difference between each other ( p < 0.05) . The survival rate of two hours, the acid resistant tests presented a higher survival rate of 85.67% ⁹8.00%, there was significant difference between PBIL1-002 and other strains ( p < 0.05) , whereas the bile salt resistance exhibited a lower survival rate of 21.17% ~ 51.20%, the difference between some strains was significant ( p < 0.05) except for PBIL1-002 and PBIL1-006.The five strains were frequently resistant to streptomycin and vancomycin, PBIL1-009 and PBIL1-018 were resistant to cotrimoxazole, PBIL1-006 and PBIL1-009 were resistant to gentamicin.The hemolysis tests showed that the five strains had no hemolytic ability.The qualitative test of amine formation showed that PBIL1-009 strain could produce spermine metabolism, PBIL1-018 strain could produce spermine and tyramine metabolism. The results suggested that the biological and safe characteristics of the different source strains were certain differences.In this way, it was necessary to select the strains with better biological and safe characteristics in the production.