接骨草总黄酮提取工艺及其抑菌作用的研究

以碱溶酸沉法研究接骨草总黄酮的提取条件和抑菌作用。以料液比、温度、时间、提取液p H为影响因素,进行单因素实验和正交实验,研究提取接骨草总黄酮的最优条件,调节提取液p H来沉淀总黄酮,并用比浊法和滤纸圆片法研究总黄酮的抑菌作用。结果表明,提取接骨草总黄酮的最优条件是料液比1∶40(g/m L)、温度为80℃、时间为45 min、提取液p H10,此条件下接骨草总黄酮的得率为3.11%。调节总黄酮提取液p H3.5沉淀接骨草总黄酮的量较多,其得率为2.15%。接骨草总黄酮在酸性p H4~5时对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的抑制作用较强,并能够增加三种细菌的电导率,对三种菌最小抑菌浓度分别为0.63、1.25、2.50 mg/m L。用碱溶酸沉法提取接骨草总黄酮的工艺简单,总黄酮具有较高的抑菌活性,为进一步开发接骨草的总黄酮打下了基础。 

Study on the extraction and antibacterial effect of total flavonoids from Sambucus chinensis Lindl

By using alkali soluble and acid sinking method, total flavonoids of Sambucus chinensis Lindl were extracted, and its antibacterial effect were studied in this paper. The extraction conditions including solid-liquid ratio, extraction temperature, extraction time, p H of xetraction liquid of flavonoids from Sambucus chinensis Lindl were optimized by single factor experiments and orthogonal experiments, and meanwhile the sinking efficiency of total flavonoids were studied by acidic p H precipitation, and the antibacterial activity of total flavonoids was studied by filter paper discs method and turbidimetric method. The results showed that the optimum extraction conditions of total flavonoids of Sambucus chinensis Lindl were as follow: solid-liquid ratio1∶ 40 ( g/m L) , extraction temperature 80 ℃, extraction time 45 min, extraction liquid p H10, in which the extraction rate of total flavonoids of Sambucus chinensis Lindl was 3.11%.Adapting the extract to p H3.5 with HCl could better precipitate about 2.15%total flavonoids of Sambucus chinensis Lindl.Total flavonoids of Sambucus chinensis Lindl had a stronger antibacterial activity on Staphylococcus aureus, Bacillus subtilis, Escherichia coli at acidic p H4 ~5 than at neutral p H and alkaline p H value, and these bacteria conductivity was increased by total flavonoids. The minimum inhibitory concentration of total flavonoids of Sambucus chinensis Lindl on three bacteria were 0.63, 1.25, 2.50 mg/m L, respectively. The extracting process of total flavonoids from Sambucus chinensis Lindl by using alkali soluble and acid sinking method was simple and inexpensive, and its total flavonoids had a great antibacterial activity, in which played a foundation on the development and utilization of total flavonoids of Sambucus chinensis Lindl.