Effect of age and cholestyramine feeding on rat liver 3-hydroxy-3-methyl glutaryl CoA reductase,sterol carrier protein 1 and sterol carrier protein 2 activities

The rate of formation of sterol from squalene in livers from suckling rats was less than one-third that of adults. This difference was due to a lesser activity of microsomal enzymes in the suckling rat livers, and not to any difference in cytosolic sterol carrier protein 1. The microsomal enzymes and sterol carrier protein 2 of the cytosol required for the conversion 7-dehydrocholesterol to cholesterol were both lower in suckling rats compared to adults. Both those activities paralleled the differences in HMG-CoA reductase activities between suckling and adult rats. Feeding of cholestryamine to adult rats increased the activities of the microsomal enzymes, sterol carrier protein 1 and sterol carrier protein 2 involved in the conversion of squalene to cholesterol.