| HPLC法测定多维片中痕量维生素D3的含量 |
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| 引用本文： | 王乔飞. HPLC法测定多维片中痕量维生素D3的含量[J]. 淮阴工学院学报, 2010, 19(3): 82-84 |
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| 作者姓名： | 王乔飞 |
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| 作者单位： | 江苏江山制药有限公司,江苏,靖江,214500 |
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| 摘 要： | 建立了运用HPLC法对痕量VD3进行含量测定的分析方法。实验中采用菲罗门硅胶(silica)柱,以异丙醇:正己烷(0.5:99.5)为流动相,流速:2ml/min,紫外检测波长:264nm,柱温:30℃。实验表明:VD3在0.1μg/ml～0.8μg/ml范围内,其色谱峰面积与质量浓度呈良好线性关系,r=0.9999(n=5),平均回收率为99.04%,进样精密度为1.26%。本法操作简便、快速,准确,适用于痕量成分的含量测定。
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| 关 键 词： | 维生素D3 高效液相色谱 含量测定 |
Determination of Trace VD3 in Multi-vitamins with HPLC |
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| WANG Qiao-fei. Determination of Trace VD3 in Multi-vitamins with HPLC[J]. Journal of Huaiyin Institute of Technology, 2010, 19(3): 82-84 |
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| Authors: | WANG Qiao-fei |
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| Affiliation: | WANG Qiao-fei ( Jiangsu Jiangshan Pharmaceuticals Co. Ltd. ,Jingjiang Jiangsu 214500,China) |
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| Abstract: | An analytical method is established for detecting trace vitamin D3 in multi-vitamins tablets with HPLC. In the experiment,the chromatographic column of Phenomenex silica is used,and hexane （ 0. 5： 99. 5） is in the mobile phase; the flow rate is 2. 0ml/min,the UV detective wavelenth is 264nm and column tempera ture is 3 0 ℃ . A good liner correlation is found between the peak areas and the concentrations in the range of 0. 1-0. 8 ug/ml of VD3（ r = 0. 9999,n = 5） . The average recovery is 99. 04% ; the precision is 1. 26% . The method proves to be rapid,accurate and applicable for the determination of the trace in vitamin D3. |
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| Keywords: | vitaminD3 HPLC determination |
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