福建省带壳牡蛎中副溶血性弧菌的市场调查

为了解零售带壳牡蛎中副溶血性弧菌(VP)的污染情况，2003年4月～2004年3月每月在福建省福州和厦门两地收集带壳牡蛎，样品共252份，分别来自水产品批发市场(11％)、零售市场(50％)和饭店(39％)。采用Vitek鉴定系统和最可能数(MPN)法进行VP的定性和定量分析。结果显示，带壳牡蛎VP几何平均密度为46MPN，100g，46％的试样VP密度低于30MPN/100g的最低检出限，仅厦门2个试样菌量超过104MPIN/100g。两个地区、不同采样点和不同季节之间试样vP平均密度差别均有显性。厦门试样菌量高于福州；批发市场试样菌量最高；春季试样菌量(93MPN/100g)高于其它季节(约为40MPN/100g)。研究结果可以用于估计生食牡蛎人群VP的暴露量。

Market survey of Vibrio parahaemolyticus in shell oysters of fujian, China

Vibrio parahaemolyticus in retail shell oysters were investigated. From April 2003 to March 2004, 252 oysters in shell were collected monthly in Fuzhou and Xiamen of Fujian Province, (39% from restaurants; 50% from retail markets and 11% from wholesale seafood markets). The V. parahaemolyticus in oyster samples was determined qualitatively and quantitatively by the Vitek identification system and the most probable number (MPN) technique. The geometric mean V. parahaemolyticus density in retail shell oysters was 46 MPN/100 g, with 46% had V. parahaemolyticus densities below the detectable level of 30 MPN/100 g; only two samples from Xiamen were found to harbor V. parahaemolyticus densities exceeding 10 4 MPN/100 g. There was significant difference in the V. parahaemolyticus densities between the two regions, and the differences were also found among different sampling sites and seasons. Oysters from Xiamen had higher densities of V. parahaemolyticus than did oysters collected from Fuzhou. The highest mean V. parahaemolyticus density was observed in samples from wholesale seafood markests. The highest mean V. parahaemolyticus density was observed in samples collected in spring (93 MPN/100 g) while approximately 40 MPN/100 g was observed in samples collected in other seasons. These data can be used to estimate the exposure of raw oyster consumer to V. Parahaemolyticus.