Direct electrochemical regeneration of enzymatic cofactor 1,4‐NADH on a cathode composed of multi‐walled carbon nanotubes decorated with nickel nanoparticles |
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| Authors: | Irshad Ali Nehar Ullah Mark A McArthur Sylvain Coulombe Sasha Omanovic |
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| Affiliation: | 1. Department of Chemical Engineering, McGill University 3610 University, Street, QC, H3A 0C5, CanadaCurrent address: Department of Chemical and Materials Engineering, University of Alberta, 11421 Saskatchewan Drive, Edmonton, AB T6G 2M9, Canada;2. Department of Chemical Engineering, McGill University 3610 University, Street, QC, H3A 0C5, Canada |
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| Abstract: | Multi‐walled carbon nanotubes (MWCNTs) were grown on a stainless steel mesh and decorated with nickel nanoparticles (Ni NPs). The developed Ni NP‐MWCNT material was then used as a cathode in an electrochemical batch reactor to electrocatalytically convert NAD+ to enzymatically‐active 1,4‐NADH. The regeneration of 1,4‐NADH was studied at various electrode potentials. At electrode potential of ?1.6 V, a very high recovery (relative amount of 1,4‐NADH in the product mixture) was obtained, 98 ± 1 %. In comparison, to achieve the same recovery on a non‐decorated MWCNT cathode, a much higher cathodic potential was needed (?2.3 V), establishing the importance of Ni NPs on the electrocatalytic activity in reducing NAD+ to 1,4‐NADH. It was postulated that hydrogen adsorbs on Ni NPs immobilized on MWCNTs to form Ni‐Hads, and this activated hydrogen rapidly reacts with neighbouring NAD‐radicals, preventing the dimerization of the latter species, ultimately yielding 1,4‐NADH. |
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| Keywords: | multiwalled carbon nanotube (MWCNT) nickel nanoparticles nicotinamide adenine dinucleotide (NADH) |
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