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玉米秸秆糖化液发酵纤维燃料体系优化研究
引用本文:宋安东,刘玉博,董庸行,杜风光.玉米秸秆糖化液发酵纤维燃料体系优化研究[J].食品与发酵工业,2008,34(12).
作者姓名:宋安东  刘玉博  董庸行  杜风光
作者单位:1. 河南农业大学生命科学学院,河南,郑州450002;河南天冠企业集团,河南,南阳,473000
2. 河南农业大学生命科学学院,河南,郑州450002
3. 河南天冠企业集团,河南,南阳,473000
基金项目:国家自然科学基金,国家农业科技成果转化资金资助项目,中国博士后科学基金,河南省高等学校青年骨干教师资助项目 
摘    要:研究了嗜鞣管囊酵母(Pachysolen tannophilus)P-01发酵玉米秸秆糖化液生产纤维乙醇的体系。在原料酶解时用磷酸缓冲液(pH4.8、0.0667mol/L KH2PO4-Na2HPO4溶液),更适合P-01发酵;玉米秸秆糖化液的发酵培养基配方为:酵母膏0.3g/L,蛋白胨0.5g/L,尿素0.2g/L,(NH4)2HPO40.1g/L,吐温800.25mmol/L,聚乙烯醇25mg/L,L-谷氨酰胺0.625g/L,pH5.0;添加油酸对发酵结果的影响不明显;在发酵至36h补料,乙醇最终体积分数为2.05%,比对照(1.54%)提高了33.17%。

关 键 词:玉米秸秆  糖化液  发酵  燃料乙醇

Research on Optimum of Fermentation System During Fermentation Corn Stalk Saccharified Liquid to Fuel Ethanol
Song Andong,Liu Yubo,Dong Yonghang,Du Fengguang.Research on Optimum of Fermentation System During Fermentation Corn Stalk Saccharified Liquid to Fuel Ethanol[J].Food and Fermentation Industries,2008,34(12).
Authors:Song Andong  Liu Yubo  Dong Yonghang  Du Fengguang
Abstract:Optimization of the fermentation conditions on corn stalk saccharified liquid to produce fuel ethanol by Pachysolen tannophilus P-01 was studied.The results showed that choosing the phosphoric acid buffer is more suitable for co-fermentation of fuel ethanol production,and the fermentation medium suitable for saccharified liquid of P-01 was selected as follows:0.3 g/L yeast extract,0.5 g/L peptone,0.2 g/L carbamide,0.1 g/L diammonium phosphate,250μmol/L tween 80,25mg/L PVA,0.625g/L L-glutamine,pH5.0.The impact of oleic acid on fermentation was weak;feeding the batch culture at 36h,the final ethanol concentration reached 2.05%,improved by 33.17%.
Keywords:corn stalk  saccharified liquid  fermentation  fuel ethanol
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