| 浓香型白酒窖泥中总DNA提取方法的研究 |
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| 引用本文： | 刘金英,汤斌. 浓香型白酒窖泥中总DNA提取方法的研究[J]. 安徽机电学院学报, 2013, 0(3): 8-11 |
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| 作者姓名： | 刘金英 汤斌 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽芜湖241000 |
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| 摘 要： | 本研究针对5种白酒窖泥总DNA提取方法（预处理＋DNA提取液＋SDS法,改进的化学裂解法,CTAB＋ SDS＋反复冻融,SDS细胞裂解法,玻璃珠＋CTAB＋溶菌酶法）,探索适合微生物多样性研究的细菌16S rDNA和真菌ITS-5.8S rDNA的PCR扩增的窖泥总DNA提取方法.结果表明,采用玻璃珠＋CTAB＋溶菌酶法和化学裂解法提取DNA无明显降解、重复性好,可用于后续细菌16S rDNA和真菌ITS-5.8S rDNA的PCR扩增.
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| 关 键 词： | 窖泥微生物 DNA提取 PCR扩增 |
Extraction of total DNA from gujing-flavor liquor pit mud |
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| LIU Jin-ying,TANG Bin. Extraction of total DNA from gujing-flavor liquor pit mud[J]. Journal of Anhui Institute of Mechanical and Electrical Engineering, 2013, 0(3): 8-11 |
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| Authors: | LIU Jin-ying TANG Bin |
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| Affiliation: | (Coll. of Biol.＆ Chem. Engn. ,Anhui Polytechnic University, Wuhu 241000, China) |
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| Abstract: | In order to extract DNA from Gujing-flavor liquor pit mud under simple and easy experimental terms for follow-up 16S rDNA and ITS-5.8S rDNA PCR analysis, five methods（pretreatment＋extraetion buffer＋ SDS method, grinding ＋ freezing ＋ thawing method, CTAB ＋ SDS ＋ freeze-- thaw method, SDS method and beads＋lysozyme＋ CTAB method） were employed and compared,it can be concluded that the beads＋lysozyme＋SDS method and grinding＋ freezing＋ thawing method are expected to be adopted as rapid and reliable protocols for further microbial molecular study of Pit Mud. |
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| Keywords: | pit mud microorganisms DNA extraction PCR amplification |
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