Screening of grapes and wine for azoxystrobin, kresoxim-methyl and trifloxystrobin fungicides by HPLC with diode array detection |
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Authors: | Susana De Melo Abreu Manuela Correia Paulo Herbert Lú cia Santos Arminda Alves |
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Affiliation: |
a Lepae-Deq, Faculdade de Engenharia da Universidade do Porto, 4200-465 Porto, Portugal |
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Abstract: | The Quinone outside Inhibitors (QoI) are one of the most important and recent fungicide groups used in viticulture and also allowed by Integrated Pest Management. Azoxystrobin, kresoxim-methyl and trifloxystrobin are the main active ingredients for treating downy and powdery mildews that can be present in grapes and wines. In this paper, a method is reported for the analysis of these three QoI-fungicides in grapes and wine. After liquid-liquid extraction and a clean-up on commercial silica cartridges, analysis was by isocratic HPLC with diode array detection (DAD) with a run time of 13 min. Confirmation was by solid-phase micro-extraction (SPME), followed by GC/MS determination. The main validation parameters for the three compounds in grapes and wine were a limit of detection up to 0.073 mg kg-1, a precision not exceeding 10.0% and an average recovery of 93% ± 38. |
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Keywords: | QoI-fungicides grapes wines method validation HPLC GC/MS |
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