Effect of diabetes and insulin replacement on the lipid properties of hepatic smooth endoplasmic reticulum |
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Authors: | Caroline Tobia Holloway Sanford A Garfield |
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Affiliation: | (1) Department of Biochemistry, University of Viriginia, 22908 Charlottesville, VA;(2) Department of Anatomy, University of Cincinnati, 45267 Cincinnati, OH |
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Abstract: | This study is a characterization of the lipid properties of the smooth and rough endoplasmic reticulum (SER, RER) of liver
from streptozotocin-induced diabetic rats. A significant decrease in membrane microviscosity was observed in the SER but not
the RER of diabetic rats when compared to that of normal controls. This decrease in SER membrane microviscosity correlated
with a decrease in cholesterol/phospholipid ratio of these membranes that could be accounted for solely by a change in the
membrane cholesterol content. Changes in phospholipid fatty acyl chain composition were also observed in the SER membranes
but these changes were small when compared to the large change in cholesterol content observed. Insulin treatment for only
one day did not significantly alter the microviscosity of the SER but after 2, 4 and 6 days of treatment both membrane microviscosity
and membrane cholesterol content were restored to values similar to those for normal animals. No significant changes in the
RER lipid composition were observed. It is well known that increases in glucose-6-Pase (G-6-Pase) activity of liver ER membranes
are associated with diabetic onset. An increase in the specific activity of G-6-Pase was observed in both SER and RER membrane
preparations, although the observed increase in the SER membrane is higher. The changes in the G-6-Pase activity of the SER
membranes were correlated with the alterations in the microviscosity and lipid composition of these membranes. It is postulated
that lipid properties of the SER membranes, may contribute to the regulation of G-6-Pase activity in that membrane. |
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