| 单点式位移平台激光共聚焦扫描荧光显微镜 |
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| 引用本文： | 陶振强,贾南南,阮斌. 单点式位移平台激光共聚焦扫描荧光显微镜[J]. 光学仪器, 2015, 37(2): 170-174 |
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| 作者姓名： | 陶振强 贾南南 阮斌 |
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| 作者单位： | 上海理工大学 光电信息与计算机工程学院, 上海 200093;上海理工大学 光电信息与计算机工程学院, 上海 200093;上海理工大学 光电信息与计算机工程学院, 上海 200093 |
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| 基金项目： | 国家自然科学基金资助项目(61178079) |
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| 摘 要： | 为了获得细胞图像,利用Visual Studio C#开发了移动位移平台的控制程序,使用位移平台单点扫描的方式设计激光共聚焦扫描显微镜(laser confocal scanning microscope,LCSM)。为了获得高分辨率的位移,位移由精度可以达到1nm的压电陶瓷驱动器驱动。设计了梳状和矩形两种扫描路径,通过程序设计位移补偿的方法弥补了机械运动的偏差。利用算术平均值的数字滤波方法处理数据采集卡采集的数据以减小随机噪声的影响。实验结果证明,利用C#程序控制的单点式平台扫描LCSM具有较好地测量效果。
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| 关 键 词： | 共聚焦 荧光显微镜 数字滤波 平台扫描 C#程序控制 位移补偿 |
| 收稿时间： | 2014-11-03 |
Single-point displacement of the platform laser confocal scanning fluorescence microscope |
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| TAO Zhenqiang,JIA Nannan and RUAN Bin. Single-point displacement of the platform laser confocal scanning fluorescence microscope[J]. Optical Instruments, 2015, 37(2): 170-174 |
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| Authors: | TAO Zhenqiang JIA Nannan RUAN Bin |
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| Affiliation: | School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China |
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| Abstract: | In order to obtain cells image, we use Visual Studio C# to develop the control program of the mobile displacement platform, and use displacement single-point scanning way to design laser confocal scanning microscope (LCSM). In order to obtain high resolution displacement, displacement of the platform with piezoelectric ceramic actuator is used and the highest accuracy can reach 1 nm. Both comb and rectangle scanning paths are designed, by programming the displacement compensation method to make up the mechanical movement deviation. Digital filtering arithmetic average method of dealing with the data acquisition card is used to collect data to reduce the influence of random noise. Experimental results show that single-point platform using C# program LCSM has a very good effect. |
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| Keywords: | confocal fluorescence microscopy digital filtering displacement of the platform C# control program displacement compensation |
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