Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors |
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| Authors: | Tata Alessandra Fernandes Anna Maria A P Santos Vanessa G Alberici Rosana M Araldi Dioneia Parada Carlos A Braguini Wellington Veronez Luciana Silva Bisson Gabriela Reis Felippe H Z Alberici Luciane C Eberlin Marcos N |
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| Affiliation: | ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas-UNICAMP , Campinas, SP, Brazil. |
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| Abstract: | The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery. |
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