静电纺再生丝素/明胶纳米纤维的结构与性能

 以质量分数为98%的甲酸为溶剂,将再生丝素与明胶以质量比70∶30进行共混静电纺丝。研究纺丝液质量分数及乙醇处理对纤维膜的结构及力学、溶解性能等的影响;测定不同纺丝液质量分数及不同厚度下纤维膜的孔隙率及孔径;在纤维膜上进行小鼠成纤维细胞(L929)和人脐静脉内皮细胞(HUVECs)培养实验。结果表明:随着纺丝液质量分数的提高或经乙醇处理后,丝素β化程度提高,纤维结晶度增大,溶失率减小,拉伸强度增大;随着纺丝液质量分数的提高,纤维膜的孔隙率减小,孔径增大,厚度增加时,纤维膜的孔隙率及孔径均减小;L929及HUVECs均能够在纳米纤维膜上黏附、生长和增殖。

Microstructure and property of electrospun regenerated silk fibroin/gelatin nanofibers

The electrospinning of regenerated silk fibroin(SF) and gelatin blended with the composition ratio 70∶30 was performed with 98% formic acid as spinning solvent.The influence of mass concentration and ethanol treatment on the microstructure,mechanical properties and solubility of electrospun SF/gelatin nanofibers was investigated.The pore diameter and porosity of SF/gelatin fiber membrane with different mass concentrations and different thickness were measured.The mouse fibroblasts and human umbilic vein endothelium cells were seed on SF/gelatin nanofibers scaffold.The results showed that: along with the mass concentrations of spinning solution increasing and ethanol treating,the content of β-sheet of SF in electrospun SF/gelatin nanofibers was increased,crystallinity increased,water-dissolved rate declined,and tensile strength enhanced of electrospun SF/gelatin nanofibers;as the mass concentration of spinning solution increased,the pore diameter of electrospun SF/gelatin nanofibers was decreased and the porosity was increased;both the pore diameter and porosity were decreased with the increase of the thickness of electrospun SF/gelatin nanofibers.The SF/gelatin nanofibers scaffold may be a good candidate for both L929 and HUVECs spreading and proliferating.