| 基因重组10BNP在大肠杆菌中的表达与纯化 |
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| 引用本文： | 刘荣,张瑛,吴蕾,蔡友庆. 基因重组10BNP在大肠杆菌中的表达与纯化[J]. 化学工业与工程, 2002, 19(1): 22-25,31 |
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| 作者姓名： | 刘荣 张瑛 吴蕾 蔡友庆 |
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| 作者单位： | 天津大学化工学院,天津,300072 |
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| 摘 要： | 在摇瓶中对基因重组脑钠素（BNP）在大肠杆菌中的表达进行了研究，确定诱导后最佳培养时间为4h，表达量达21．8％，采用金属螯合亲和层析法对十聚体脑钠素进行了纯化，比较了咪唑洗脱法和降pH值洗脱法对目标蛋白的纯化效果，得到了一条纯化目标蛋白最佳亲和层析纯化路线，为后续实验打下了很好的基础。
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| 关 键 词： | 脑钠素 重组大肠杆菌 金属螯合亲和层析 基因重组脑钠素 BNP 表达 纯化 |
| 文章编号： | 1004-9533(2002)01-0022-04 |
Expression and Purification of 10BNP Engineering Bacteria |
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| LIU Rong,ZHANG Ying,WU Lei,CAI You-qing. Expression and Purification of 10BNP Engineering Bacteria[J]. Chemical Industry and Engineering, 2002, 19(1): 22-25,31 |
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| Authors: | LIU Rong ZHANG Ying WU Lei CAI You-qing |
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| Abstract: | Expression of recombinant 10BNP in E.coli was studied in shake flasks and the optimum induction time is 4h.The expression level was over 21 8%.10BNP fusion protein which was produced as inclusion body (IB) was separated by breaking bacteria through sonication.IB was properly dissolved in the presence of 8M Urea.By means of Immobilized metal ion affinity chromatograph(IMAC),comparing the results of different elution methods,a optimum purification route was attained.The work facilitates the further research. |
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| Keywords: | brain natriuretic peptide recombinant Easherichia coli Immobilized metal ion affinity chromatograph(IMAC) |
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