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| 植物甜蛋白des-pGlul-brazzein在大肠杆菌中表达条件的优化 | |
| 引用本文： | 李春丽,陈其新,何国庆. 植物甜蛋白des-pGlul-brazzein在大肠杆菌中表达条件的优化[J]. 食品与发酵工业, 2006, 32(7): 8-11 |
| 作者姓名： | 李春丽 陈其新 何国庆 |
| 作者单位： | 1. 河南农业大学牧医工程学院,郑州,450002 2. 河南农业大学牧医工程学院,郑州,450002;甘肃亚盛集团博士后科研工作站北京分站,北京,100101 3. 浙江大学生物系统工程与食品科学学院,杭州,310019 |
| 基金项目： | 河南省教育厅基础研究项目;河南工业大学校科研和教改项目 |
| 摘 要： | 构建了植物甜蛋白des-pGlul-Brazzein高效表达的工程菌株,对其IPTG诱导条件进行了研究。IPTG浓度、诱导时间和诱导温度对菌株生长和目的蛋白表达的试验结果显示,温度是重要的影响因素。进一步的研究表明,菌株在28~30℃以0.2mmol/LIPTG诱导4h,对菌株生长和目的蛋白的表达有利,目的蛋白的表达可达到总蛋白的43.8%。 |
| 关 键 词： | 植物甜蛋白 大肠杆菌 高效表达 优化 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006-03-272006-06-02 |
Optimizing Culture Conditions for the Expression of des - pGlul - Brazzein in E. coli BL21 (DE3) Pljys |
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| Li Chunli,Chen Qixin,He Guoqing. Optimizing Culture Conditions for the Expression of des - pGlul - Brazzein in E. coli BL21 (DE3) Pljys[J]. Food and Fermentation Industries, 2006, 32(7): 8-11 | |
| Authors: | Li Chunli Chen Qixin He Guoqing |
| Affiliation: | 1.College of Animal and veterinary Science, Henan Agricultural University,Zhengzhuo 450002, China; 2.Beijing Branch of Postdoctoral Workstation of Gansu Yasheng Group Company,Beijing 100101, China;3.College of Biosystem Engineering and Food Science,Zhenjiang University,Hangzhuo 310029, China |
| Abstract: | Expression strain of des-pGlul-brazzein was constructed and the conditions used IPTG as inducer was also optimized.The effect of three factors which were IPTG concentration,induction time and temperature on growth of strain and on the yield of des-pGlul-brazzein was studied in detail.The result indicated that temperature was the most important factor.Further analysis showed the greater expressed level of des-pGlul-Brazzein,as high as 43.8% of total cell protein,could be gained when stain was induced by 0.2mmol/L IPTG under 28～30℃ for 4h. |
| Keywords: | des - pGlul- brazzein E. coli recombinant expression optimization |
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