利用糖蜜废水产乳酸细菌的分离与鉴定

为获得可利用糖蜜废水产生乳酸的细菌，采用厌氧Hungate培养技术，从糖蜜废水处理生物反应器中获得活性污泥，分离到一株乳酸菌MD-9。对该株细菌进行了形态学特征、生理生化指标、16S rRNA基因序列分析等研究。结果表明与最相近的种属Lactobillus fermnetus的16S rRNA基因序列同源性为99.6%。该株细菌为兼性厌氧杆菌，葡萄糖代谢特征为专性异型发酵，可以利用糖蜜废水产生乳酸。

Isolation and identification of lactic acid bacterium using molasses wastewater to produce lactic acid

In order to obtain lactic acid bacteria using molasses wastewater to produce lactic acid,an activated sludge was sampled from bioreactor with molasses wastewater,and strain MD-9 was isolated by an anaerobic Hungate technique.The morphology,physic-biochemical characteristics and 16S rRNA sequence analysis of strain MD-9 were investigated.The results indicated that 16S rRNA gene homology of strain MD-9 with Lactobacillus fermentum was 99.6%.Strain MD-9 is facultative anaerobe bacillus.Glucose fermentation is heterologous.Strain MD-9 can use molasses wastewater to produce lactic acid.