| 高效液相色谱电喷雾质谱联机检测黑豆异黄酮 |
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| 引用本文： | 李丹,牟莉,李晓磊,谭克.高效液相色谱电喷雾质谱联机检测黑豆异黄酮[J].食品科学,2007,28(9):438-441. |
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| 作者姓名： | 李丹 牟莉 李晓磊 谭克 |
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| 作者单位： | 1. 长春大学吉林省农产品加工重点实验室,吉林,长春,130022;国家大豆深加工技术研究推广中心,吉林,长春,130022
2. 长春大学吉林省农产品加工重点实验室,吉林,长春,130022 |
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| 基金项目： | 科技部科技成果重点推广项目;吉林省科技发展计划 |
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| 摘 要： | 采用C18色谱柱,以甲醇:水:甲酸30min内,0.8ml/min梯度洗脱(从20:80:0.1到80:20:0.1)黑豆70%乙醇提取液。紫外光谱和电喷雾质谱的数据表明:大豆苷、乙酰基染料木苷、染料木苷、丙二酰基大豆苷、丙二酰基黄豆苷和丙二酰基染料木苷是黑豆种子中最主要的异黄酮,总含量为0.373%,为普通大豆种子异黄酮含量的2～6倍;黑豆是异黄酮的丰富来源。
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| 关 键 词： | 黑豆 异黄酮 高效液相色谱 质谱 紫外光谱 |
| 文章编号： | 1002-6630（2007）09-0438-04 |
| 修稿时间： | 2006-07-18 |
Identification and Determination of Black Bean Isoflavones by High Performance Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrum |
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| LI Dan,MU Li,LI Xiao-lei,TAN Ke.Identification and Determination of Black Bean Isoflavones by High Performance Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrum[J].Food Science,2007,28(9):438-441. |
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| Authors: | LI Dan MU Li LI Xiao-lei TAN Ke |
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| Affiliation: | 1.Jilin Key Laboratory of Agricultural Products Processing, Changchun University, Changchun 130022, China ; 2.National Research and Development Center for Soybean Processing Technology, Changchun 130022, China |
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| Abstract: | Mobile phases of 100% methanol/water/formic acid from 20:80:0.1 to 80:20:0.1 respectively in 30 min were used to elute black bean isoflavones at 0.8 ml/min,extracted by 70% ethanol from mature black bean seeds,in a C18 column with a high performance liquid chromatography(HPLC)system coupled with diode array detector(DAD)and mass spectrum(MS).Daidzin,acetyl genistin,genistin,malonyl daidzin,malonyl glycitin and malonyl genistin are identified as the main isoflavones with a total content 0.373%.Black bean is a good source of isoflavones for human health. |
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| Keywords: | black bean isoflavone HPLC MS UV |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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