Association study of single nucleotide polymorphisms in IL-10 and IL-17 genes with the severity of microbial keratitis |
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| Affiliation: | 1. School of Optometry and Vision Science, University of NSW, Sydney 2052, Australia;2. UCL Institute of Ophthalmology, University College London, EC1V 9EL, UK;3. Moorfields Eye Hospital NHS Foundation Trust, London, EC1V 2PD, UK;4. UCL Genetics Institute, University College London, WC1E 6BT, UK;5. William Harvey Research Institute, Clinical Pharmacology, Queen Mary University of London, London, EC1M 6BQ, UK;1. A-Infection, Genomic, Immunology and Cancer Research Group, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;2. Center of Biotechnology and Biodiversity Research and Development, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;3. Department of Parasitology, Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;4. Department of Microbiology, Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta, Indonesia;1. Centro de Finanzas, IESA, Venezuela;2. Colegio de Estudios Superiores de Administración-CESA, Colombia;3. Idalion Capital Group, USA;1. Department of Ophthalmology, New Zealand National Eye Centre, The University of Auckland, New Zealand;2. School of Optometry and Vision Science, New Zealand National Eye Centre, The University of Auckland, New Zealand;1. Eye Research Center, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Optometry, Iran University of Medical Sciences, Tehran, Iran;3. Noor Research Center for Ophthalmic Epidemiology, Noor Eye Hospital, Tehran, Iran;4. Department of Optometry, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran;5. Department of Foreign Languages, Tehran University of Medical Sciences,Tehran, Iran;6. Refractive Errors Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;7. Mashhad University of Medical Sciences, Mashhad, Iran;8. Department of Medical Surgical Nursing, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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| Abstract: | PurposeExploratory analysis to assess the association of single nucleotide polymorphisms (SNPs) in the interleukin (IL) 10 and IL-17 genes with severity of contact lens keratitis.MethodsThis was a retrospective case control study of 88 contact lens keratitis cases (25 severe) and 185 healthy contact lens wearers recruited from studies conducted at Moorfields Eye Hospital and in Australia-wide during 2003–2005. Buccal swab samples were collected on Whatman FTA cards and mailed by post for DNA extraction and SNP genotyping. IL-10 (rs1800871; rs1800896; rs1800872) and IL-17 (rs1800871; rs1800896; rs1800872) SNPs were screened by pyrosequencing. Genetic association analyses were performed via Cochran-Armitage trend tests and logistic regression models using PLINK software.ResultsNone of the SNPs tested showed evidence of association with severity of contact lens keratitis at P < 0.05. Nevertheless, minor allele G in SNP rs2397084 of the IL-17F gene was associated with increased risk of severe MK, with OR=2.1 (95% CI=0.9-4.8, P = 0.066).ConclusionOur study cannot exclude with confidence that genetic variation in the IL-17 F proinflammatory cytokine is associated with more severe outcomes of MK. However, there is general body of information that the IL-17 pathway is important in the mechanisms of MK. Studies with larger power and the expanded array of laboratory tools will elucidate the exact role of IL-17 in MK. |
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| Keywords: | Keratitis Interleukin Gene Contact lens |
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