Evaluation estrogen,progesteron and androgen receptor expressions in corneal epithelium in keratoconus |
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| Affiliation: | 1. Samsun Carsamba State Hospital, Department of Ophthalmology, Samsun, Turkey;2. Ankara Ataturk Education and Research Hospital, Department of Ophthalmology, Ankara, Turkey;3. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Medical Biology, Ankara, Turkey;4. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Pathology, Ankara, Turkey;5. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Ophthalmology, Ankara, Turkey;6. Hacettepe University, Bioengineering, Ankara, Turkey;1. Optometry and Vision Department, Faculty of Optics and Optometry, Complutense University of Madrid, Madrid, Spain;2. Ophthalmology Department, San Carlos University Hospital, Complutense University, Madrid, Spain;3. Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, Wroclaw, Poland;1. School of Optometry, Tianjin Vocational Institute, China;2. School of Optometry, Nova Southeastern University, The United States;3. College of Optometry, Pacific University, India;1. Contact Lens Services, Tej Kohli Cornea Institute, L V Prasad Eye Institute, Hyderabad, India;2. Brien Holden Institute of Optometry and Vision Sciences, L V Prasad Eye Institute, Hyderabad, India;3. Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, India;1. University of Milano Bicocca, Materials Science Department, via R. Cozzi 55, I-20125 Milan, Italy;2. University of Milano Bicocca, Department of Biotechnology and Bioscience, Piazza della Scienza 2, I-20126 Milan, Italy;3. University of Milano Bicocca, COMiB Research Centre, via R. Cozzi 55, I-20125 Milan, Italy;4. School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK |
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| Abstract: | PurposeTo evaluate the role of sex steroid hormone receptors in corneal epithelium in etiopathogenesis of keratoconus (KC).MethodsThirty patients with KC who were planned for corneal collagen-crosslinking and 20 patients who were planned for excimer laser for refractive errors included in this study. Corneal epitheliums were curated mechanically during surgeries. Right eyes were evaluated immunohistochemically and left eyes were evaluated by quantitative polymerase chain reaction (qPCR) to investigate estrogenα, estrogenβ, progesterone and androgen receptors.ResultsImmunohistochemically, staining for progesterone and androgen receptors did not significantly differ between KC and control groups (p > 0.05). None of the cases had staining for estrogenα and estrogenβ receptors. qPCR showed that mRNA expressions of estrogenα and androgen receptors were significantly higher in the KC group (p < 0.001).ConclusionA significantly higher rate of estrogenα and androgen receptor expressions in corneal epithelium from patients with KC through qPCR supports a possible relation between KC and sex steroid hormones. |
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| Keywords: | Keratoconus estrogen progesterone androgen receptor corneal epithelium |
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