Enrichment of polyunsaturated fatty acids from sardine cannery effluents by enzymatic selective esterification |
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Authors: | Murielle Schmitt-Rozieres Valérie Deyris Louis-Claude Comeau |
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Affiliation: | (1) Laboratory of Applied Biological Chemistry, Faculty of Science of Saint-Jerome Case 431, Ave. Escadrille Normandie-Niemen, 13397 Marseille Cedex 20, France |
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Abstract: | The sardine canning industry produces vast quantities of effluents that need expensive reprocessing. Their oily component contains valuable n−3 polyunsaturated fatty acids, namely EPA (5,8,11,14,17-eicosapentaenoic acid) and DHA (7,10,13, 16,19-docosahexaenoic acid), up to 10% each. Our aim was to develop a process allowing the recovery of these fatty acids. After removing solid particles, proteins, and peptides from the crude effluent, the obtained oil was hydrolyzed. EPA and DHA were enriched from the recovered free fatty acid fraction by selective enzymatic esterification. Lipases were used as biocatalysts: LipozymeTM allowed up to 80% DHA enrichment but gave no EPA enrichment. By immobilizing Candida rugosa lipase on Amberlite IRC50 cation-exchange resin, a 30% EPA enrichment was obtained. |
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Keywords: | Candida rugosa docosahexaenoic acid eicosapentaenoic acid fatty acid enrichment fish oil immobilized enzyme lipase polyunsaturated fatty acid Rhizomucor miehei |
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