The fatty acid and aldehyde composition of the major phospholipids of mouse brain |
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Authors: | Grace Y Sun Lloyd A Horrocks |
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Affiliation: | (1) Laboratory of Neurochemistry, Cleveland Psychiatric Institute, Cleveland, Ohio |
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Abstract: | Phospholipid classes were separated from mouse brain lipid extracts by preparative thin-layer chromatography (TLC). Methyl
esters were prepared from the intact phospholipids by direct transesterification at room temperature in the presence of silica
gel by using 0.5m NaOH-methanol in order to prevent interference by aldehydes or derivatives. Dimethyl acetal derivatives of phosphoglyceride
alkenyl ethers (alkenyl moiety with a double bond in 1,2-position relative to oxygen linkage) were prepared, using 5% concentrated
HCl in methanol, followed by preparative TLC for isolation.
The major phospholipids present were ethanolamine phosphoglycerides (EPG) 39.8%, choline phosphoglycerides (CPG) 39.7%, serine
phosphoglycerides (SPG) 15.0%, and sphingomyelin (Sph) 5.4%. One-fifth of the total phospholipids (PL) were in the form of
plasmalogens, mainly EPG. Choline and serine plasmalogens were present in trace quantities. The major aldehyde components
of the plasmalogens were 16∶0, 18∶0, and 18∶1.
The EPG were rich in long-chain poly-unsaturated fatty acids, including 28.8% of 22∶6 and 17.0% of 20∶4, but contained only
7.2% of 16∶0. In contrast, the CPG contained 39.6% of 16∶0, and 31.0% of 18∶1 with a small content of polyunsaturated fatty
acids. The SPG exhibited a still different pattern containing 38.2% of 18∶0, 23.2% of 18∶1, 24.3% of 22∶6, 2.9% of 16∶0, and
3.8% of 20∶4.
Presented in part at the AOCS Meeting, New Orleans, May 1967. |
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