Electrogenerated chemiluminescence. 83. Immunoassay of human C-reactive protein by using Ru(bpy)3(2+)-encapsulated liposomes as labels |
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Authors: | Zhan Wei Bard Allen J |
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Affiliation: | Department of Chemistry and Biochemistry, University of Texas, 1 University Station, A5300, Austin, Texas 78712, USA. |
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Abstract: | Liposomes ( approximately 100-nm diameter) containing Ru(bpy)32+ (bpy = 2,2'-bipyridine) were prepared as an electrogenerated chemiluminescent (ECL) tag for a sandwich-type immunoassay of human C-reactive protein (CRP). Polyclonal human CRP antibodies were introduced onto liposomes and magnetic beads through biotin-streptavidin interaction. The antigen-antibody conjugates formed on addition of a CRP-containing sample were separated from unreacted species magnetically. Addition of 0.1 M tri-n-propylamine and 0.1 M phosphate buffer (pH 7.6) containing 0.1 M NaCl and 1% (v/v) Triton X-100 caused liberation of the Ru(bpy)32+ from the liposome. ECL obtained in this medium showed a detection limit of 100 ng/mL for human CRP with good linearity of ECL intensity versus antigen concentration over the range 100 ng/mL-10 microg/mL. |
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