|
|||||
|
|
| 大肠杆菌定量检测用标准物质的研制 | |
| 引用本文： | 薛蕾,林婧,隋志伟,王晶,刘晓夏,傅博强,张玲,郝林.大肠杆菌定量检测用标准物质的研制[J].计量学报,2015,36(6):652-656. |
| 作者姓名： | 薛蕾 林婧 隋志伟 王晶 刘晓夏 傅博强 张玲 郝林 |
| 作者单位： | 1. 中国计量科学研究院, 北京 100029 2. 山西农业大学食品科学与工程学院, 山西 太谷 030800 3. 南京市计量监督检测院, 江苏 南京 210049 |
| 基金项目： | 国家质检总局食品安全专项（ASPAQ1304）;国家高技术研究发展计划 (2014AA021406) |
| 摘 要： | 采用平板计数法考察复合保护剂对大肠杆菌标准物质冻干粉的保护效率以及均匀性、稳定性。结果显示:复合保护剂对大肠杆菌保护率达到53.19%,可以在冻干过程中有效保护菌体。将大肠杆菌与冻干保护剂混合后,经冷冻干燥制备成干粉状标准物质,形态好、复水速度快、均匀性良好,可以稳定保存半年。该标准物质可用于食品检测实验室质量控制和食品中大肠杆菌检测结果的评价。 |
| 关 键 词： | 计量学 大肠杆菌 冷冻干燥 标准物质 复合保护剂 |
Preparation of Reference Material for Quantitative Detection of Escherichia Coli |
|
| XUE Lei,LIN Jing,SUI Zhi-wei,WANG Jing,LIU Xiao-xia,FU Bo-qiang,ZHANG Ling,HAO Lin.Preparation of Reference Material for Quantitative Detection of Escherichia Coli[J].Acta Metrologica Sinica,2015,36(6):652-656. | |
| Authors: | XUE Lei LIN Jing SUI Zhi-wei WANG Jing LIU Xiao-xia FU Bo-qiang ZHANG Ling HAO Lin |
| Affiliation: | 1. National Institute of Metrology, Beijing 100029, China; 2. College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030800, China; 3. Nanjing Institute of Measurement and Testing Technology, Nanjing, Jiangsu 210049, China |
| Abstract: | The protective effect of freeze-dried protectant, homogeneity and stability of the escherichia coli reference material are verified by plate count method. The results show that the protection of the composite protective agent reach 53.19%, and it can effectively protect the escherichia coli during freeze-drying. After escherichia coli and the freeze-dried protectant mixed, frozen, dried and prepared a dry powder reference materials of escherichia coli,its shape, rehydration speed and homogeneity are good, and it can be stable for six months. The reference material can be used to quality control of food testing laboratory and evaluation of food test results. |
| Keywords: | metrology escherichia coli freeze-drying reference material composite protective agent |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《计量学报》浏览原始摘要信息 | |
| 点击此处可从《计量学报》下载全文 | |