| 纳豆菌分离、鉴定及纳豆激酶高产菌株的正向选育 |
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| 引用本文： | 方祥,周换彩,王忠霞,孙丽娜,李彬. 纳豆菌分离、鉴定及纳豆激酶高产菌株的正向选育[J]. 食品与发酵工业, 2005, 31(12): 26-29 |
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| 作者姓名： | 方祥 周换彩 王忠霞 孙丽娜 李彬 |
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| 作者单位： | 华南农业大学食品学院,广州,510642 |
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| 摘 要： | 从日本纳豆食品中分离到1株枯草芽孢杆菌纳豆亚种(Bacillus subtilis natto)Bs01-1菌株,根据纳豆芽孢杆菌产纳豆激酶与蛋白酶活性的正相关关系,采用紫外线直接照射涂菌蛋白平板进行诱变育种的,成功地筛选到2株突变株MBS04-6和MBS04-9,其溶纤活性分别比出发菌株提高了87%和69%,达到4 179 IU/mL和3788 IU/mL。该法筛选菌株的正向突变率达到10%。
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| 关 键 词： | 枯草芽孢杆菌纳豆亚种 纳豆激酶 突变率 诱变育种 |
| 收稿时间： | 2005-09-20 |
| 修稿时间： | 2005-09-20 |
Isolation and Forward Breeding of Bacillus subtilis natto with High Fibrinolytic Activity Nattokinase |
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| Fang Xiang,Zhou Huancai,Wang Zhongxia,Sun Lina,Li Bin. Isolation and Forward Breeding of Bacillus subtilis natto with High Fibrinolytic Activity Nattokinase[J]. Food and Fermentation Industries, 2005, 31(12): 26-29 |
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| Authors: | Fang Xiang Zhou Huancai Wang Zhongxia Sun Lina Li Bin |
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| Affiliation: | College of Food Science, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Bacillus subtilis natto Bs01-1 strain was isolated from Natto, a Japanese traditional fermented soy food. According to the relationship of the proteinase activity and fibrinolytic activity of nattokinase, two mutants, MBS04-6 and MBS04-9 were selected from Bs01-1 strain by the UV radiation. The fibrinolytie activities of nattokinase from of these two mutants were up to 4179IU/mL and 3788IU/mL,respeetively. The forward mutation radio of Bacillus subtilis natto with nattokinase fibrinolytie activity was 10 %. |
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| Keywords: | Bacillus subtilis natto nattokinase fibrinolytic activity mutation radio mutation breeding |
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