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| X射线辐照后人肝细胞、肝癌细胞存活与端粒酶活性关系的研究 | |
| 引用本文： | 刘金婷,党秉荣,李文建,荆西刚,魏巍,王转子,丁楠,高清祥. X射线辐照后人肝细胞、肝癌细胞存活与端粒酶活性关系的研究[J]. 辐射研究与辐射工艺学报, 2010, 28(2): 119-123 |
| 作者姓名： | 刘金婷 党秉荣 李文建 荆西刚 魏巍 王转子 丁楠 高清祥 |
| 作者单位： | 1. 中国科学院近代物理研究所,兰州,730000;中国科学院研究生院,北京,100049 2. 中国科学院近代物理研究所,兰州,730000 3. 兰州大学生命科学院,兰州,730000 |
| 摘 要： | 利用X射线对体外培养的人肝癌细胞SMMC-7721和正常人肝细胞HL-7702进行辐照,以细胞克隆形成法测定细胞存活率,多聚酶链式反应银染端粒重复序列扩增法(Telomeric repeat amplification protocol,TRAP-PCR)检测细胞中端粒酶活性变化,探讨辐照后细胞存活与细胞中端粒酶活性变化的关系。结果表明,SMMC-7721和HL-7702细胞的存活率都随辐照剂量的增加而相应降低。在1-4Gy剂量范围内,两种细胞的端粒酶活性变化均呈剂量依赖性增强。但在1Gy、2Gy、3Gy剂量下,SMMC-7721其端粒酶活性较对照(0Gy)细胞低;当辐照剂量达4Gy时,端粒酶活性略高于对照细胞。在1-4Gy剂量范围内,HL-7702和SMMC-7721的细胞存活与端粒酶活性在变化趋势上呈负的关系。 |
| 关 键 词： | X射线 细胞存活率 端粒酶活性 SMMC-7721 HL-7702 |
The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays |
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| LIU Jinting,DANG Bingrong LI Wenjian JING Xigang WEI Wei WANG Zhuanzi DING Nan GAO Qingxiang. The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays[J]. Journal of Radiation Research and Radiation Processing, 2010, 28(2): 119-123 | |
| Authors: | LIU Jinting DANG Bingrong LI Wenjian JING Xigang WEI Wei WANG Zhuanzi DING Nan GAO Qingxiang |
| Affiliation: | (Institute of Modern Physics,Chinese Academy of Science,Lanzhou 730000,China) 2(Graduate Schol of Chinese Academy of Science,Beijing 100049,China) 3(School of Life Sciences,Lanzhou University,Lanzhou 730000,China) |
| Abstract: | In this paper,the relationship between cell survival fraction and telomerase activity was investigated with the human hepatocellular cells HL-7702 and carcinoma cells SMMC-7721 irradiated by X-rays. The colony formation assay and TRAP （Telomeric repeat amplification protocol） method were used to determine the cell survival fraction and telomerase activity respectively. To both of HL-7702 and SMMC-7721,the telomerase activity was increased in dose-dependent. However,at 1—3 Gy,the telomerase activity was lower than that of control in SMMC-7721,and it was a little higher than that of control when irradiated at 4 Gy. At 1—4 Gy,the variation trend of cell survive and that of telomerase activity were identical. |
| Keywords: | SMMC-7721 HL-7702 |
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